Metagenomic analysis of Hot Springs of Surajkund and the exploration of thermostable enzymes from endogenous isolates

Abstract

Hot springs serve as a hub for microbial diversity, the microflora of the hot springs can be a source of numerous biologically important molecules. Extremophile bacteria were isolated from different hot springs of India to investigate their potential in industrial applications. The analysis involved both culture dependent and culture independent methods. The metagenomic analysis of the microflora was carried out using next-gen sequencing. Also, thermophilic isolates were culture and primarily screened for amylase, xylanase and cellulase enzyme production. The production for amylase was optimized and partial purification was carried out. Partially purified amylase was tested and characterized using; UPLC, DLS-ZP and TGA. The retention time for the amylase was observed to be around 0.5 minutes along with a stable thermo gravimetric profile around 400oC confirming its stability at higher temperatures. One of the thermophilic isolate was able to degrade lignocellulosic waste exhibiting the potential of isolates from the kund.