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In this study, we particularly aimed to research the effect of supplemental humic acid on ruminal fermentation and blood variables in rams. A trial was conducted to evaluate the effect of humic acid (HA) on protozoa count, percentages of different protozoa types and blood parameters. Three male Kivircik rams with ruminal cannula were used in a Latin square design, during 22 days periods (15 days for adaptation, 7 days for collection). They received 0 control group (CG), 5 g/day or 10 g/day of HA (HA5, HA10, assay groups). HA were added to the ration with grain diet. Ration was consisted of 5% grain diet and 95 % alfalfa hay. Rumen contents collected before and, 3h and 6h after morning feeding on days 1 and 7 in each collection period were analyzed. Blood samples were also collected the same days. No significant difference in biochemical and hematological parameters (except eosinophils levels, P<0.05), variables of ruminal fluid (except sodium levels before feeding) and species of rumen protozoa organism (except the percentage Epidinium spp.) were evidenced with the addition of HA.In conclusion, we think that they might have been true the widely use in animal food of humates which don't have any negative effect on biochemical and hematological parameters in particular.

Out on the next frontier of nutritional research will be the complete biochemical and physiological characterization of plant-derived foods that prevent or delay the development of chronic diseases in humans and animals. The chemical composition of many major crop products (seeds, flour, oil, leaves, etc.) have been determined, but the slow process of evaluating each compound alone or in mixtures for the biological function in nutrition and health of the animals that consume them has only just begun. Camelina, or false flax ( Camelina sativa L. Crantz), is an emerging oil seed crop in North America mostly used as a biodiesel fuel. The seeds contain up to 45 % oil, which is rich in polyunsaturated omega-3 and omega-6 fatty acids, as well as fat-soluble antioxidants such as the vitamin E-active tocopherols. Extraction of oil from camelina seeds by mechanical expeller yields a seed meal that consists of approximately 10 % residual oil, 45 % crude protein, 10 % soluble sugars, 13 % fiber, 5 % minerals, and 10 % phytochemical constituents such as glucosinolates, flavonols, lignans, phenolic acids as well as nucleic acids. The seed meal also contains a hydrophilic gum. While the oil fraction has been well characterized and its uses are growing, the seed meal has yet to be fully characterized for its potential use Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.