Expansion in whole genome sequencing and subsequent increase in antibiotic resistance targets have paved the way of high throughput qPCR (HT-qPCR) for analyzing hundreds of antimicrobial resistance genes (ARGs) in a single run. A meta-analysis of 51 selected studies is performed to evaluate ARGs abundance trends over the last 7 years. WaferGenTM SmartChip is found to be the most widely used HT-qPCR platform among others for evaluating ARGs. Up till now around 1000 environmental samples (excluding biological replicates) from different parts of the world have been analyzed on HT-qPCR. Calculated detection frequency and normalized ARGs abundance (ARGs/16S rRNA gene) reported in gut microbiome studies have shown a trend of low ARGs as compared to other environmental matrices. Disparities in the HT-qPCR data analysis which are causing difficulties to researchers in precise interpretation of results have been highlighted and a possible way forward for resolving them is also suggested. The potential of other amplification technologies and point of care or field deployable devices for analyzing ARGs have also been discussed in the review. Our review has focused on updated information regarding the role, current status and future perspectives of HT-qPCR in the field of antimicrobial resistance.

In this research, a new single-phase direct AC-to-AC converter, operating in buck and boost mode, with a bipolar voltage gain, is proposed. The operation is accomplished through high frequency direct and indirect PWM control of a single switch with low voltage stresses. This reduces, not only the control effort, but also the switching losses. The low voltage stresses across the high frequency switches, reduce the dv/dt problem significantly without any loss and bulky voltage snubber arrangement. The operation, in its all-operating modes, has a low inductor ripple current and switching current. The proposed converter may be employed as an AC voltage restorer in a power distribution system to cope with the voltage sag and swell issues. The detailed analysis of the proposed converter is carried out in order to compare its performance with the existing converters. The simulation results obtained using the MATLAB/Simulink environment are verified through experimental results.

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