Ultrathin-layer isoelectric focusing in 100 pm polyacrylamide gels is described for the separation of the isozymes of the PGMl-locus. The PGM1-subgroups are best resolved in pH 5-7 gradients obtained by mixing Ampholine and Servalyt carrier ampholytes in a 2:l ratio. By using a high field strength (180 V/cm) in the final stage of isoelectric focusing, the isozyme bands are sharper than in conventional thin-layer gels. This facilitates identification of the subgroups. Distortions of the pH gradient are avoided if low initial field strengths (15-20 V/cm) are used. The method is highly reproducible and equally applicable for the analysis of blood samples as well as blood stains.
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