Application of high-performance thin-layer chromatography and automated multiple development for the identification and determination of pesticides in water

Vigne, Ulf De La; Jänchen, D.; Weber, Walter H.

doi:10.1016/s0021-9673(01)88520-4

Cited by 27 publications

(2 citation statements)

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“…As an alternative separation method to liquid chromatography, thin-layer chromatography (TLC) could be used. The separation efficiency of TLC is lower compared to HPLC but was improved by the development of new materials for the stationary phase , and new techniques for plate development like automated multiple development (AMD) which allows a sequential development of a thin-layer plate with solvents of lower elution strength resulting in a gradient elution of the analytes. , With the overpressured layer chromatography (OPLC) a technique is available which combines the principles of TLC and high-performance liquid chromatography (reviewed in refs and ).…”

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Direct Coupling of Thin-Layer Chromatography with a Bioassay for the Detection of Estrogenic Compounds: Applications for Effect-Directed Analysis

et al. 2013

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The present study investigated the hypothesis that the coupling of high-performance thin-layer chromatography with the yeast estrogen screen (planar-YES, p-YES) can be used as a screening tool for effect-directed analysis. Therefore, the proposed method was challenged for the first time with several real samples from various origins such as sediment pore water, wastewater, and sunscreens. It was possible to detect and quantify estrogenic effects in all investigated sample types, even in the presence of demanding matrixes. Furthermore, the specific agonistic effect of the estrogen receptor activation could be detected in samples exhibiting cytotoxic effects and at cytotoxic levels of analyzed estrogenic compounds, which is not possible with the classic YES. The analysis of samples by the p-YES results in profiles of estrogenic activity. By means of this profiles samples can be compared qualitatively and quantitatively with respect to different compositions of bioactive compounds in mixtures. In conclusion, the p-YES approach seems to have a high potential to be used as a valuable screening tool for various applications in effect-directed analysis.

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Direct Coupling of Thin-Layer Chromatography with a Bioassay for the Detection of Estrogenic Compounds: Applications for Effect-Directed Analysis

et al. 2013

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“…Traditionally, pesticide residue analysis has been undertaken mainly by gas chromatography (GC) coupled to sensitive and selective detectors such as electron-capture [6,7], nitrogen-phosphorous [8], and mass-spectrometric [9] detectors, although the use of thin layer chromatography (TLC) [10,11] and immunoassay techniques [12] has been also reported.…”

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Multiresidue Analysis of Phenylurea Herbicides in Environmental and Drinking Water by Capillary Electrophoresis

Barroso

Konda²,

Morovján³

1999

J. High Resol. Chromatogr.

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The present paper describes the application of capillary electrophoresis in the micellar mode to the separation and quantitative determination of five phenylurea herbicides, viz. monuron, linuron, diuron, isoproturon, and monolinuron, in water samples. Using uncoated fused silica capillary and phosphate‐borate buffer (pH 7.0) containing SDS, the five pesticides are resolved in less than 15 min and quantitatively determined by an ultraviolet detector at 244 nm. Method optimization and validation parameters are presented. Good linearity and repeatability were observed for all the compounds studied (correlation coefficients 0.999). The feasibility of the method developed was tested by simultaneous determination of these herbicides in environmental and drinking water samples at the minimum residue levels (MRLs) (0.1 μg/L) after solid‐phase extraction (SPE) preconcentration procedure.

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Organochlorine, Pyrethrin and Pyrethroid Insecticides: Single Class, Multiresidue Analysis of

Muccio

2000

Encyclopedia of Analytical Chemistry

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Organochlorine (OC) pesticides is a general term for a broad class of compounds including some well‐known compounds such as dichlorodiphenyltrichloroethane (DDT), lindane, dieldrin, endrin, heptachlor epoxide (HEPO), endosulfan, and chlordane. OC pesticides are the first class of compounds introduced in agricultural and civil uses to counteract noxious insects and insect‐borne diseases. In general they are lipophilic compounds with noticeable chemical and environmental stability. Although most OC pesticides have been progressively restricted and then banned in the 1970s in most industrialized countries, a widespread environmental pollution has resulted from their use in agriculture and civil uses. Pyrethrins are natural insecticidal compounds found in the extract of pyrethrum flowers. Also, a number of pesticides have been synthesized that share the biologically relevant chemical moiety with the pyrethrins and are referred to as pyrethroids (PYR). These compounds possess equal or better biological activity and better chemical stability that allow their use in agriculture, husbandry, and civil applications. Both OC and PYR pesticides can occur in fruits and vegetables as well as in food of animal origin and environment. The levels can be different as a result of direct application or indirect contamination. Such different matrices and levels of contamination require different analytical approaches and resources. A review is presented of the array of the analytical techniques most frequently applied for the extraction, cleanup and identification/determination. Extraction techniques include solvent extraction, supercritical fluid extraction (SFE), solid‐phase extraction (SPE), and solid‐phase microextraction (SPME). Solvent extraction is carried out in different ways depending on the type of matrix (fatty and nonfatty samples, soil, sediments, and water), such as Soxhlet, liquid–liquid extraction (LLE), pressurized liquid extraction (PLE), and matrix solid‐phase dispersion (MSPD). Different cleanup techniques are presented that can be applied to fatty and nonfatty samples. They include liquid– liquid partitioning (LLP), size‐exclusion chromatography (SEC), sweep codistillation and assisted distillation, column adsorption chromatography, chromatography on SPE cartridges, high‐performance liquid chromatography (HPLC), thin‐layer chromatography (TLC) and degradative cleanup. The techniques are presented as modular steps that can be arranged in different ways to cope with cleanup requirements posed by different sample composition and different selectivity/sensitivity of the identification/determinative techniques. The performances of the cleanup steps are discussed in terms of matrix removal and amount of sample that can be handled. Also the possibilities of linking different steps and the chance for automation of the cleanup process are indicated. Some environmental pollutants, such as polychlorinated biphenyl (PCB), have similar analytical behavior to some OC pesticides and can occur in the same environmental matrices. Some cleanup steps useful for the group separation of PCB and OC pesticides are presented. The techniques for identification and determination include gas chromatography (GC) with selective detectors, HPLC, and their combination with mass spectrometry (MS). The importance of the combination of responses from different analytical techniques to obtain reliable identification at trace levels is underlined.

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Application of high-performance thin-layer chromatography and automated multiple development for the identification and determination of pesticides in water

Cited by 27 publications

References 1 publication

Direct Coupling of Thin-Layer Chromatography with a Bioassay for the Detection of Estrogenic Compounds: Applications for Effect-Directed Analysis

Direct Coupling of Thin-Layer Chromatography with a Bioassay for the Detection of Estrogenic Compounds: Applications for Effect-Directed Analysis

Multiresidue Analysis of Phenylurea Herbicides in Environmental and Drinking Water by Capillary Electrophoresis

Organochlorine, Pyrethrin and Pyrethroid Insecticides: Single Class, Multiresidue Analysis of

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