An efficient protocol was developed for large scale propagation using seed as an explant in Withania somnifera. MS supplemented with BAP at 0.6 mg/l with 0.4 mg/l IAA was found to be most effective in initiating multiple shoots at the rate of ten per explant. By repeated subculturing, a high frequency of shoot multiplication was established. About 90% rooting was achieved with 0.4 mg/l of IBA and 0.4 mg/l IAA. Micropropagated plants were hardened in half strength of MS and then established in (1 : 1) sand and soil mixture.Withania somnifera is known by common names such as Winter cherry, Indian ginseng, belonging to Solanaceae, mainly cultivated in a limited area of Mandsore district of M.P. However, it is widely distributed throughout the dry regions of India up to an altitude of 2000 m in Himalayas. Withania somnifera contains flavonoids and many active ingredients of the class withanolides. So far 12 alkaloids, 35 withanolides and several sitoindosides have been isolated from this plant species and thoroughly studied for its medicinal properties. Much of the pharmacological activity of Withania somnifera has been attributed to two main withanolides, withferin A and withanolide.Withaferin A is the most important of the withanolide isolated from Withania somnifera, to which the curative properties of the leaves are attributed (Uma and Akagi 1996, Mohan 2004). Studies over two decades indicate that withanolide has anti-inflammatory, anti tumor, antistress (Archana and Namasivayam1999), antioxidant, mind boosting, rejuvenating and antimicrobial properties (Jaffer and Jawad 1988). Withanolides also account for multiple medicinal applications. In addition to the alkaloids, the roots are reported to contain starch, reducing sugars, hentriacontane, glycosides, dulcitol, and withaniol.Withania somnifera can be propagated both by sexual and asexual method. Seed propagation, however is not always satisfactory, since the heterogenetically the strain produces a great deal of variation. Again multiplication through cuttings give rise to less ramified plants and is consequently less productive than plants obtained from seeds. The requirement of Withania somnifera has sharply raised due to its popularity owing to a large scale unrestricted exploitation. This

Momordica charantia commonly known as bitter melon/gourd, a member of Cucarbitaceae. Qualitative phytochemical analysis of Momordica charantia confirms the presence of photochemicals like flavonoids, saponins, terpenoids, coumarins, emodins, alkaloids, proteins, etc. HPLC is a simple, accurate and selective technique for separation and quantification of anthraquinone and flavonoid and phenols from leaf sample of bitter gourd. The most common method for chromatographic determination of anthraquinone is the HPLC method. Anthraquinones are a class of natural products encompassing several hundreds of compounds, differing in the nature and positions of substituent groups. Many anthraquinones have potential therapeutic value, since antimicrobial, insecticidal, antitumor, anticongestive, hypotensive and sedative properties have been assigned to these compounds.