HPLC Method for Analysis of Bioactive Compound fromMomordica charantia

Abstract

Momordica charantia commonly known as bitter melon/gourd, a member of Cucarbitaceae. Qualitative phytochemical analysis of Momordica charantia confirms the presence of photochemicals like flavonoids, saponins, terpenoids, coumarins, emodins, alkaloids, proteins, etc. HPLC is a simple, accurate and selective technique for separation and quantification of anthraquinone and flavonoid and phenols from leaf sample of bitter gourd. The most common method for chromatographic determination of anthraquinone is the HPLC method. Anthraquinones are a class of natural products encompassing several hundreds of compounds, differing in the nature and positions of substituent groups. Many anthraquinones have potential therapeutic value, since antimicrobial, insecticidal, antitumor, anticongestive, hypotensive and sedative properties have been assigned to these compounds.

<em>Momordica charantia</em> extract modulates inflammatory response in human lymphocytes via suppression of TNF-α

“…The amount of quercetin found in the MCE sample also was reported in other studies. The work of Pereira (18) related that quercetin was the main flavonoid found and Supe et al (19) demonstrated that the concentration of quercetin was between 0.05 to 0.2% in the analyzed sample. Sathasivam et al (20) also found kaempferol in high amounts in the fruit extract of MCE, corroborating our findings.…”

A comprehensive review on phytochemistry, nutritional and pharmacological properties of Momordica charantia

“…36 There are several studies proving that different parts of M. charantia contain antimicrobial activity (Table 1). 34,37 According to Wang et al 38 the antimicrobial activity of M. charantia is assigned to its content of seed oil, tannins, triterpenoids, alkaloids, antimicrobial protein, steroids, and cardiac glycolysis.…”

The Potential of Bitter Melon Residues Concerning its Physico-chemical Characterization, Bioactive Compounds, and Antioxidant Effects

“…[3][4][5] Phytochemical analysis of M. charantia revealed the presence of saponins, flavonoids, terpenoids, coumarins, emodins, and alkaloids. [6] Conventional techniques of phenolic extraction are generally associated with high consumption of organic solvent and long extraction times. With increasing environmental pollution caused by the larger use of volatile and harmful organic solvents, recent techniques on extraction focusing on minimum usage of solvents or use alternative bio-rational solvents.…”