Which is very rich in protein in plant Lemna minor L. abundant in Turkey ecologically plays a very important role in protecting the elimination of water pollution and aquaculture environment balance. In this study, in vitro propagation of this plant with Temporary Immersion System Bioreactor and determination of the effects of used plant growth regulators on the protein content of the plant were aimed. With this objective different variant of media with and without sucrose, varying pH and concentrations of BAP, kinetin, TDZ in medium were analyzed. Experiments for micropropagation were performed for 8 hours in the dark and 16 hours in white fluorescent light (150 µ mol photons m-2s-1) under photoperiod and at 24 ± 1 ° C. The highest plant growth was observed at pH 7.23 in sugar free liquid MS medium containing 0.2 mg/L BAP. 50.44 plants per explant were recorded in this medium. In addition, the maximum number of plants per explant in liquid MS medium containing 0.05 mg/L kinetin was calculated as 57,823 and the maximum number of plants per explant in liquid MS medium containing 0.6 mg/L TDZ was calculated as 50.74. As a result of nitrogen determination studies with Kjeldahl method, the protein value of the plant was determined as 25.5%. As a result of hormone application, it was seen that protein content in plant increased to 29.18% with 0.5 mg/L BAP. It was concluded that the aim of the study were fulfilled and positive effects of Temporary Immersion System Bioreactors on plant multiplication were found.
In vitro amplification of the nucleic acids (DNA or RNA) is used in the detection of microbial agents and thus in the diagnosis of infectious diseases, as well as in the diagnoses of oncological and genetic disorders and forensic medicine. The aim of the present study was to compare the isolation methods of the nucleic acids of hepatitis B and C viruses, causative agents of the two significant infections worldwide. Conventional isolation methods were compared with the commercial kits that have been used commonly in recent years, in terms of reliability, cost-effectiveness, contamination risk and duration of the testing time. Five standards for the isolation of the viral nucleic acids of both HBV DNA (Fluorion HBV QNP 2.0) and HCV RNA (Fluorion HCV QNP 2.1) were used. The isolations of the viral nucleic acids of HBV and HCV were done with the conventional methods, phenol-chloroform and guanidine thiocyanate, and the commercial kits Roboscreen and NucleoSpin. The resultant viral nucleic acid load was determined with a spectrophotometer (WPA UV 1101, Biotech Photometer), and their amplification was conducted with Real-Time PCR. The results of the assessments revealed that the highest nucleic acid concentration were obtained with the conventional methods, while they exhibited significant drawbacks such as long duration of the testing time, difficulty in application, and higher contamination risk.
SummaryThis study presents a method for the production of rhamnolipid, a biosurfactant, by Pseudomonas sp. Pseudomonas sp. cells that were grown in nutrient agar were inoculated into sterile liquid medium. Following an incubation period of 24 h, 2 ml of cells were inoculated into a different liquid medium and the results were obtained at the end of 26 hours incubation time. In our study, the effects of temperature, pH, and glucose concentration on rhamnolipid production were also investigated. Later, the same procedure was applied to immobilized cells that were kept away from the free microorganisms. The production of rhamnolipid by free cells was found to be much higher than that of immobilized cells. Free cells could be used for rhamnolipid production effectively. Keywords: Biosurfactant, Rhamnolipid, Pseudomonas sp., Immobilized cells, Free cells Serbest ve Tutuklanmış Pseudomonas sp. Hücrelerinden Ramnolipid (Biyosürfektan) Eldesi ÖzetBu çalışmada, bir büyosürfektan olan ramnolipidin Pseudomonas sp. 'den üretimi araştırılmıştır. Nutrient agarda üretilen Pseudomonas sp. örnekleri sıvı besiyerine aktarılmıştır. 24 saatlik inkübasyon sonrası, 2 ml'lik kültür örnekleri ramnolipid üretimi için, farklı bir sıvı besiyerine aktarılarak 26. saatte sonuçlar gözlenmiştir. Çalışmada, ayrıca ramnolipid üretimine sıcaklığın, pH'ın ve glukoz konsantrasyonunun etkisi de incelenmiştir. İmmobilize (tutuklanmış) hücreler içinde aynı metotlar uygulanmıştır. Ramnolipid üretimi, serbest hücrelerde tutuklanmış hücrelerden daha yüksek bulunmuştur. Serbest hücreler, etkin ramnolipid üretimi için kullanılabilirler.
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