Farklı Koşullar Altında Bacillus subtilis ATCC 6633 Tarafından Poli-β-Hidroksibütirat (PHB) Üretiminin Araştırılması

Tamdoğan, Nazan; Sıdal, Uğur

doi:10.9775/kvfd.2011.2474

Cited by 3 publications

(3 citation statements)

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“…We observed maximum cell growth as well as PHB accumulation at 48 h. After 48 h, the cell vitality reduced, and the PHB contents decreased by almost 50% compared to the level at 48 h. Initiation of PHB degradation when the cells reached the stationary phase was reported previously, confirming the importance of harvesting time on PHB production [3]. Similar to our results on the effects of temperature, PHB synthesis decreased at higher and lower temperatures than 30 • C in Bacilllus subtilis, which is likely caused by the decline of PHB synthetic enzyme activity [46]. PHB production is significantly affected by nitrogen availability when the amount of carbon remains the same and is not required under nutrient-rich conditions [5,47], supporting our data on the effects of the carbon/nitrogen ratio in the medium.…”

Section: Discussionsupporting

confidence: 91%

Molecular Profiling and Optimization Studies for Growth and PHB Production Conditions in Rhodobacter sphaeroides

et al. 2020

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In the recent climate change regime, industrial demand for renewable materials to replace petroleum-derived polymers continues to rise. Of particular interest is polyhydroxybutyrate (PHB) as a substitute for polypropylene. Accumulating evidence indicates that PHB is highly produced as a carbon storage material in various microorganisms. The effects of growth conditions on PHB production have been widely studied in chemolithotrophs, particularly in Rhodobacter. However, the results on PHB production in Rhodobacter have been somewhat inconsistent due to different strains and experimental conditions, and it is currently unclear how diverse environmental factors are linked with PHB production. Here, we report optimized growth conditions for PHB production and show that the growth conditions are closely related to reactive oxygen species (ROS) regulation. PHB accumulates in cells up to approximately 50% at the highest level under dark-aerobic conditions as opposed to light aerobic/anaerobic conditions. According to the time-course, PHB contents increased at 48 h and then gradually decreased. When observing the effect of temperature and medium composition on PHB production, 30 °C and a carbon/nitrogen ratio of 9:1 or more were found to be most effective. Among PHB biosynthetic genes, PhaA and PhaB are highly correlated with PHB production, whereas PhaC and PhaZ showed little change in overall expression levels. We found that, while the amount of hydrogen peroxide in cells under dark conditions was relatively low compared to the light conditions, peroxidase activities and expression levels of antioxidant-related genes were high. These observations suggest optimal culture conditions for growth and PHB production and the importance of ROS-scavenging signaling with regard to PHB production.

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Section: Discussionsupporting

confidence: 91%

Molecular Profiling and Optimization Studies for Growth and PHB Production Conditions in Rhodobacter sphaeroides

et al. 2020

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“…The PHB production was generally high at neutral levels in the study in which the effect of the pH was investigated (Figure ). It was reported that the maximum PHB yield was observed at pH 7 in the culture of Bacillus subtilis . In a similar study, the optimum pH value in PHB production was found as 6.5.…”

Section: Discussionmentioning

confidence: 51%

“…In the study in which we investigated the effect of the incubation temperature on PHB production, the maximum PHB yield was determined at 35°C (Figure ). It has been reported in the literature that the PHB production with the maximum yield occurred under mesophilic conditions, and the highest PHB yield at these temperatures were significant for industrial applications …”

Section: Discussionmentioning

confidence: 99%

Polyhydroxybutyrate and hydroxyvalerate production by Bacillus megaterium strain A1 isolated from hydrocarbon‐contaminated soil

Güngörmedi

Demirbilek

Mutlu

et al. 2014

J of Applied Polymer Sci

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In this study, we attempted to find an alternative microbial resource as a bioplastic producer. Among all of the isolates, the A1 strain produced 44% poly(β‐hydroxybutyrate) (PHB) in proportion to its dry cell weight. The molecular identification of the 16S RNA gene showed that this bacterium was a strain of Bacillus megaterium with the accession number KC579390. The optimization studies led us to the conclusion that the highest poly(β‐hydroxybutyrate‐co‐hydroxyvalerate) (PHBV) production was 78% when 5% molasses was used as the carbon source at pH 6 and 35°C after 60 h of incubation. Attenuated total reflectance Fourier transform infrared (FTIR) spectroscopy and H‐NMR were used for chemical characterization. Differential scanning calorimetry was used to determine the thermal properties of the PHB and PHBV that were synthesized with sucrose and molasses as carbon sources, respectively. The FTIR spectra of the polymers were characterized by typical absorption bands at 1715–1720 cm−1 for amide‐bound CO bands and 1261–1279 cm−1 for an ester‐bound CO band. The molecular weights of PHB and PHBV synthesized with sucrose and molasses were calculated as 428 and 498 kDa, respectively, according to the viscometric method. This study indicated that the B. megaterium strain A1 is an alternative microbial resource as a bioplastic producer. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40530.

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Extraction and Characterization of Polyhydroxybutyrate (PHB) From Bacillus flexus MHO57386.1 Isolated From Marine Sponge Oceanopia arenosa (Rao, 1941)

Aryaraj

Pramitha

2021

Marine Science and Technology Bulletin

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Polyhydroxybutyrate (PHB) is the most widely studied biodegradable plastic that does not release any toxins or residues in the environment like petroleum based plastics. This work has been undertaken to screen PHB accumulating microorganisms from marine sponges and a total of sixteen isolates were collected and purified. Screening of isolated strains was done by Nile blue staining and observed under Leica LSCM to confirm the production of PHB. Yellow pigmented AB8a isolate from Oceanopia arenosa scored positive for PHB accumulation and subjected to morphological, biochemical and phylogenetic characterization. The biopolymer was extracted by dispersion of sodium hypochlorite and chloroform solution and characterized by FT-IR and 1H NMR for the confirmation as PHB. The highest PHB production (70.25% /100 ml) was achieved at pH 7.0 by applying dextrose as medium at incubation temperature 30°C and 150 rpm agitation speed. The FTIR spectrum of the PHB sample showed major peaks at 3457, 1692, 1550, 1454, 1420, 1190 and 1050 cm-1 , whereas the remaining peaks are closely laid between 3450 cm-1 and 600 cm-1. 1 H NMR spectrum of PHA isolated from dextrose media indicated characteristic signals of PHB. The spectrum also revealed the presence of three groups of signals characteristic of PHB by the doublet at 1.3 ppm attributed to the methyl group coupled to one proton; and the spectrum of the quadruplet at 2.57 ppm, the methylene group adjacent to an asymmetric carbon atom bearing a single proton and the multiplet at 5.28 ppm indicated signals of PHB. The PHB accumulated bacterium identified as Bacillus flexus strain based on characterization studies and 16S rRNA sequence analysis and confirmed the presence of intracellular accumulated polymer substantiated as PHB.

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Farklı Koşullar Altında Bacillus subtilis ATCC 6633 Tarafından Poli-β-Hidroksibütirat (PHB) Üretiminin Araştırılması

Cited by 3 publications

References 0 publications

Molecular Profiling and Optimization Studies for Growth and PHB Production Conditions in Rhodobacter sphaeroides

Molecular Profiling and Optimization Studies for Growth and PHB Production Conditions in Rhodobacter sphaeroides

Polyhydroxybutyrate and hydroxyvalerate production by Bacillus megaterium strain A1 isolated from hydrocarbon‐contaminated soil

Extraction and Characterization of Polyhydroxybutyrate (PHB) From Bacillus flexus MHO57386.1 Isolated From Marine Sponge Oceanopia arenosa (Rao, 1941)

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