The heptafluorobutyryl derivatives of some steroid standards were analyzed on a 25 m X 0.32 mm OV-1 fused silicacolumn. The effluent was split in the ratio : FID 5 : ECD 1 by using split arms of 20 cm X 0.32 mm ID and 20 X 0.25 mm ID respectively. Make-up gas was nitrogen with a flow-rate of 22 ml min-'. The amount injected was 1 ng per compound corresponding to approximately 160 pg for ECD detection. Interesting in this analysis is the increasing ECD response with increasing fluorine content of the derivatives.The response of El (peak 2), E2 (peak l), E3 (peak3), and E4 (peak 8) containing respectively one, two, three, and four heptafluorobutyryl groups is therefore approximately 1 :2:3:4.Thisallows easy identification of these compounds in complex biological fluids. The response of these compounds in the FIB trace is equal.
ConclusionMultiple detector operations can easily be performed with polyimide split devices. The devices described are suitable for numerous applications.
AcknowledgmentsWe thank the "Ministerie voor Wetenschapsbeleid", the "Nationaal Fonds voor Wetenschappelijk Onderzoek -NFWO" and the "lnstituut voor Wetenschappelijk Onderzoek in Nijverheid en Landbouw -IWONL" for financial aid to the laboratory.
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MS
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