Implant-related infection is one of the serious problems in regenerative medicine.Promising approach to overcome the problems caused by bacterial growth on the medical implants is their modification by bioactive coatings. A versatile technique for designing multilayer films with tailored characteristics at the nanometer scale is layer-by-layer assembly. In this study, multilayer films based on biopolymers (pectin and chitosan) and their nanocomposites with silver nanoparticles have been prepared and evaluated. The buildup of multilayers was monitored using the quartz crystal microbalance with dissipation technique. The morphology of the obtained films was investigated by atomic force microscopy. We have demonstrated that pectin-Agcontaining films were characterized by the linear growth and smooth defect-free surface. When pectin-Ag was substituted for the pectin in the multilayer systems, the properties of the formed coatings were significantly changed: the film rigidity and surface roughness increased, as well as the film growth acquired the parabolic character. All prepared multilayer films have shown antibacterial activity against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria.The significant decrease in the number of the adhered E. coli on the multilayer surface has been determined; moreover, many of the cells were misshapen with cytoplasm leaking. The prepared multilayer films showed a mild activity against S. aureus predominantly due to the antiadhesive effect. Our results indicate that antibacterial activity of biopolymer multilayers is determined by the film composition and physicochemical characteristics and can be associated with their antiadhesive and bactericidal behaviors.
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