Physicochemical aspects of design of ultrathin films based on chitosan, pectin, and their silver nanocomposites with antiadhesive and bactericidal potential

Abstract

Implant-related infection is one of the serious problems in regenerative medicine.Promising approach to overcome the problems caused by bacterial growth on the medical implants is their modification by bioactive coatings. A versatile technique for designing multilayer films with tailored characteristics at the nanometer scale is layer-by-layer assembly. In this study, multilayer films based on biopolymers (pectin and chitosan) and their nanocomposites with silver nanoparticles have been prepared and evaluated. The buildup of multilayers was monitored using the quartz crystal microbalance with dissipation technique. The morphology of the obtained films was investigated by atomic force microscopy. We have demonstrated that pectin-Agcontaining films were characterized by the linear growth and smooth defect-free surface. When pectin-Ag was substituted for the pectin in the multilayer systems, the properties of the formed coatings were significantly changed: the film rigidity and surface roughness increased, as well as the film growth acquired the parabolic character. All prepared multilayer films have shown antibacterial activity against gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria.The significant decrease in the number of the adhered E. coli on the multilayer surface has been determined; moreover, many of the cells were misshapen with cytoplasm leaking. The prepared multilayer films showed a mild activity against S. aureus predominantly due to the antiadhesive effect. Our results indicate that antibacterial activity of biopolymer multilayers is determined by the film composition and physicochemical characteristics and can be associated with their antiadhesive and bactericidal behaviors.

Chitosan-Based Antibacterial Films for Biomedical and Food Applications

“…Recently elaborated chitosan films with silver nanoparticles demonstrated high antibacterial activity against E. coli. At the same time, the prepared multilayers showed mild activity against S. aureus predominantly due to the antiadhesive effect, and this material looks promising as a prospective material for covering the surface of medical implants in order to reduce their bacterial contamination [ 58 ]. Chitosan-based dressing hydrogel films with AgNPs, elaborated Karami et al showed strong antibacterial activity against Bacillus cereus and Staphylococcus aureus and a positive effect on tissue regeneration [ 59 ].…”

Evaluation of the multifunctional activity of silver bionanocomposites in environmental remediation and inhibition of the growth of multidrug-resistant pathogens

“…57,58 For example, the Ag NP-loaded chitosan/pectin nanocomposite demonstrated exceptional antibacterial capacity for both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria due to synergistic effects. 59 Ag/Fe 3 O 4 /starch nanocomposites were endowed with high antibacterial activity against E. coli with low minimum inhibitory concentration. 60 The antimicrobial activity of Ag NPs is due to the high surface area, extra small size, and high ion release rate.…”

“…60 The antimicrobial activity of Ag NPs is due to the high surface area, extra small size, and high ion release rate. 59 The extra small and highly stable Ag NPs disrupt the cell membrane and bind with macromolecules such as DNA, RNA, and proteins, destroy them and finally cause cell death. 60 In this study, we have reported the synthesis of cellulosesupported Ag bionanocomposites (AGC NCs) by using an ecofriendly method.…”