L-ascorbic acid (C1), L-ascorbyl-2-sulfate (C 2), and L-ascorbyl-2-monophosphate (C3-M) were each incorporated into a laboratory -made P . monodon diet. Diets were extracted by deionized water or by 5% meta-phosphoric acid and then subjected to standing for 0 , 10, 20, 30, 40, 50, and 60min before high performance liquid chromatography (HPLC) analysis. Results indicated that the laboratory diet -making process destroyed about 67-75% of C1 and 21-24% of both C2 and C3-M . C2 and C3-M were stable before the analysis after either the water or meta-phosphoric acid extraction . On the other hand, a steady decline of 27. 8, 41.1, 62.6, 73.9, 81.7, and 85 .7% in C1 activity was found when extracted by water and allowed to stand for 10 , 20, 30, 40, 50, and 60min, respectively, before the HPLC analysis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.