A simple Reverse phase liquid chromatographic method has been developed and subsequently validated for the derivative of the Phenyl substituted derivatives on the hydroxyl proton of Eugenol. The separation was carried out using a mobile phase consisting of 0.5 %v/v Acetic Acid buffer in HPLC grade water (Mobile phase A) and Methanol (Mobile phase B). The column Inertsil ODS, 3V (C18, 5 μ, 25 cm × 4.6 mm id with flow rate of 1.0 ml / minute using PDA detection at 260 nm. The described method was linear over a concentration range of 10-100 μg/ml (ppm). All the five mixtures are well separated and the resolution complies as per the pharmacopeia method development requirements. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of the said derivatives of 4-allyl-2-methoxyphenyl 3,4dimethoxybenzoate (RLS-228), 4-allyl-2-methoxyphenyl 3,4,5-trimethoxybenzoate (RLS-222), (Z)-4-allyl-2-methoxyphenyl 3-(4-methoxyphenyl) acrylate (RLS-205), 4-allyl-2-methoxyphenyl 4-ethoxy-3-methoxybenzoate (RLS-193) and 4-allyl-2-methoxyphenyl benzoate (RLS-218
This paper describes the extraction of embeline from its plant source. The embelin is highly handy in the medical management viz., cytotoxic, antibacterial, wound healing, chemo preventive, lipid lowering agent and many other medical usages. The substituted benzoyl ester derivative of the embelin molecule will also have the medical properties more or less then the parent embelin molecule. They substituted derivatives were synthesized by reacting embelin with different substituted benzoyl halides in the presence of pyridine as base and dichloromethane as solvent. Amongst them, Fluoro, bromo and Iodo (halides) substituted benzoate esters exhibited different thermal activities then its parent embeline molecule. These substituted benzoyl halides showed different thermal activities. These properties are varying with respect to the position of halide in the phenyl substituted ring. The thermal prosperities played vital role in altering lipid lowering activity of the molecule.
A simple reverse phase liquid chromatographic method has been developed and subsequently validated for the derivative of the Embelin Esters substituted derivatives. The separation was carried out using a mobile phase consisting of acetic acid buffer in HPLC grade water (as mobile phase A) and methanol (as mobile phase B). An Inertsil ODS, 3.0 V (250 X 4.6 mm and 5.0 µ), HPLC column was used with flow rate of 1.0 ml / min using UV-Vis detection at 260 nm of Shimadzu HPLC (model: LC-2010). The analytical method, data integrations, processes and calculations were processed by LC solution soft ware. There are ten Embelin substituted ester derivatives are taken for analysis viz., RLS 33, 34, 36, 37, 38, 40, 41, 42, 44 and 45. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of the ten derivatives interims of validation parameters viz., separation, system suitability, System Precision and linearity in a single simple HPLC run.
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