Effect of high pressure carbon dioxide treatment (35˚C, 6.5 MPa) (HPCT) on the heat tolerance of bacterial spores was investigated. Spores of Bacillus coagulans and B. licheniformis were subjected to HPCT following heat treatment (80, 85 and 90˚C for 30 min) (HT). Spores of both types were sterilized completely in 30 min-90˚C HT after HPCT. These results indicated that HPCT greatly decreased the heat resistance of spores.
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