Dentine hypersensitivity is a short and sharp pain from exposed dentine in response to thermal changes, tactile stimulation, osmotic and chemical stimulation. Chitosan is reportedly capable of closing the dentinal tubules to prevent hypersensitivity resulting from pain stimulation. The study aimed to evaluate the ability of nano chitosan silver nitrate in the desensitization of dentinal tubular hypersensitivity. A total of 24 odontectomy third molars were divided into three groups: control, nano chitosan-silver nitrate, and varnish gel (5% sodium fluoride -tricalcium phosphate). Next, the dentinal tubules are examined by SEM, and EDS analyzes the chemical elements. The three test materials' ionic properties were reviewed with a conductivity meter, while the viscosity used a viscometer. Chitosan-silver nitrate gel showed a better ability to close dentine tubules than varnish gel at intervals of 24 and 48 hours (p>0.05). Chitosan can increase calcium compounds in closing dentine tubules than varnish gel, particularly at 48 hours. Furthermore, both chitosan's conductivity and oxygen solubility values were higher than the varnish gel.: Chitosan has a high intensity in closing the dentinal tubules in in-vitro studies of dentine hypersensitivity, which correlates with increased calcium on dentine's surface by showing better solubility ionic conductivity and oxygen dissolves.
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