Sintrong merupakan satu jenis tanaman yang banyak dikonsumsi sebagai lalapan oleh masyarakat sunda. Sintrong diketahui memiliki kandungan senyawa polifenol yang memiliki potensi sebagai antibakteri. Pada penelitian ini dilakukan penetapan kadar polifenoldan uji aktivitas antibakteri dari ekstrak etanol daun sintrong. Penetapan kadar polifenol dilakukan dengan metode Jeong et al (2005) menggunakan reagen Folin-Ciocalteu dengan asam galat sebagai standar. Uji aktivitas antibakteri dilakukan dengan metode difusi agar. Hasil pengujian diperoleh kadar total senyawa fenolik ekstrak etanol daun sintrong adalah 1,8581 g GAE/100 g ekstrak dan konsentrasi hambat minimum ekstrak terhadap Escherichia coli ATCC 89391 sebesar 8% setara dengan konsentrasi Tetrasiklin HCl 8,698 µg/ml serta konsentrasi hambat minimum terhadap Staphylococcus aureus ATCC 65381 sebesar 8% setara dengan konsentrasi Tetrasiklin HCl 11,913 µg/ml.
Gowok atau Kupa (Syzygium polycephalum) merupakan buah asli Indonesia. Pada tanaman gowok, umumnya bagian yang dimanfaatkan adalah buahnya. Namun keberadaanya kini mulai langka dan sampai saat ini, penelitian mengenai buah gowok dalam kaitannya sebagai antioksidan belum pernah dilakukan. Antioksidan adalah senyawa kimia yang dapat menyumbangkan satu atau lebih elektron kepada radikal bebas, sehingga radikal bebas tersebut dapat direndam. Antioksidan sintetik seperti BHA (Butil Hidroksi Anisol), BHT (Butil Hidroksi Toluen), PG (Propil Galat) dan TBHQ (Tert-Butil Hidrokuinon) dapat menyebabkan karsinogenesis, hal ini menyebabkan penggunaan antioksidan alami mengalami peningkatan. Antioksidan alami dalam buah dan sayuran yang memiliki fungsi seperti mencegah berkembangnya radikal bebas di dalam tubuh sekaligus memperbaiki sel-sel tubh yang rusak. Tumbuh-tumbuhan diketahui kaya akan antioksidan misalnya vitamin C, beta karoten, Vitamin E, dan flavonoid. Ekstraksi simplisia buah kupa ini dilakukan dengan metode maserasi menggunakan pelarut etanol 95%. Uji aktivitas antioksidan dilakukan secara kualitatif dan kuantitatif. Uji kualitatif antioksidan dilakukan dengan Kromatografi Lapis Tipis menggunakan pengembang n-heksan : etanol (6:4) untuk daging buah dan etil asetat : methanol (8:2) untuk biji, serta menggunakan penampak bercak 1-1-difenil-2 pikrilhidrazil (DPPH) 0,2%. Uji kuantitatif antioksidan buah kupa dilakukan dengan metode DPPH dengan vitamin C sebagai pembanding. Hasil pengujian menunjukkan ekstrak biji buah lebih aktif menunjukan aktivitas antioksidan dan termasuk kedalam antioksidan sangat kuat (IC 50 5,246 ppm) serta ekstrak daging buah termasuk kedalam antioksidan kuat (IC 50 60,187 ppm).
The 1-(4-hexylbenzoyl)-3-methylthiourea compound has been successfully synthesized by reacting 4-hexylbenzoyl chloride and 1-methylthiourea via the reflux method using a triethylamine catalyst. The 1-(4-hexylbenzoyl)-3-methylthiourea compound was identified by UV-visible, FT-IR, 13 C/ 1 H-NMR and Mass spectrophotometry. From the activity test on four cancer cell lines (HeLa, T47D, WiDr and MCF7 cell), it could be seen that it had better activity on four cancer cells than the control, hydroxyurea.
Tuberculosis (TB) is a chronic disease as a result of Mycobacterium tuberculosis. It can affect all age groups, and hence, is a global health problem that causes the death of millions of people every year. One of the drugs used in tuberculosis treatment is isonicotinohydrazide (Isoniazid). In this study, N'-benzoylisonicotinohydrazide derivative compounds (a-l) were prepared using acylation reactions between isonicotinohydrazide and benzoyl chloride derivatives, employing the reflux method. Molecular docking studies suggested that all of the compounds had better interaction with Mycobactarium tuberculosis enoyl-acyl carrier protein reductase (InhA) than isonicotinohydrazide. It can be concluded that N'-benzoylisonicotinohydrazide derivatives (a-l) can be used as anti-tuberculosis candidates. The docking results obtained revealed that all of the compounds were interacted well with InhA, with compound g exhibiting the best interaction.
Despite the long and wide application, traditional medicine is known for its minimum efficacy. Nanoparticle technology has reported to optimally address this weakness by enhancing the ability of the medicine to penetrate the biological membrane and, thereby, increasing the absorption. In this research, Etlingera elatior (ginger flower) extract, which has antioxiandt activity, was formulated into nanoparticles with ionotropic gelation method using chitosan (0.08, 0.09, 0.1, 0.2, and 0.3%) and 0.01% NaTPP. The nanoparticles were characterized by their particle size, polydispersity index, zeta potential, and entrapment efficiency against total phenolic compound, flavonoid, and anthocyanin. The ones with the best properties were then analyzed with Scanning Electron Microscopic (SEM) method and tested for its antioxidant activity against DPPH. The results showed that all of the formula variations produced particle size in the range of 147.0-566.2 nm with a polydispersity index of < 0.5 and zeta potential between 0.45-45.90 mV. Also, the absorption efficiencies of phenol, flavonoid, and anthocyanin were 72.62-84.24%, 55.18-92.05%, and 75.67-97.96%, respectively. Overall, the best characteristics were presented by the combination of 0.1% chitosan and 0.01% NaTPP, which produced 246.4-nm nanoparticles with a polydispersity index of 0.418, and zeta potential of 26.60 mV. These nanoparticles also contained phenol, flavonoid, and anthocyanin with good absorption efficiencies, namely 78.5186%, 92.05%, and 97.96%, respectively. SEM analysis showed that these nanoparticles were round and had a soft surface. The radical scavenging activities of the extract and the nanoparticles against DPPH, as presented by the IC 50 values, were 19.614 ppm and 160 ppm.
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