Human blood was stored for 8 to 9 weeks in polyethylene storing bags, and the effect of addition of adenine or adenine and inosine to the storing ACD medium was investigated.When both inosine and adenine were added (AT-blood), normal ATP level was maintained throughout the storage, while the level was falled to as low as one tenth of the normal value when the blood was stored in the usual ACD medium.The addition of only the former compound (A-blood), somewhat protected the ATP level against declination. The effect was, however, much less distinguished than the AT-blood.The characteristic discoidal shape was also maintained in the AI-blood.Thus, our previous results, obtained from experiments with usual glass storage bottles, were cconfirmed using polyethylene resavoir bags.
When a red cell suspension is prepared by suspending un-washed red cells, it may happen that the minute quantities of serum adhering to the cells cause an unexpectedly great change in nature of the suspension and lead to intricacy in studying the interaction between the cells and the artificial medium. By the way, a lasting effect of pretreatment of red cells with saline solution is also observed by several researchers. The irreversible change of washed red cells subjected to its hemolysability in hypo-or hypertonic saline solution, as stated by Kuroda (1936), Ponder (1948) etc., is a case in point.Miyake (1955) also observed the irreversible transformation of non-nucleated erythrocyte, previously washed with the saline solution.In the study on S.L.I.R. of red cell suspension the above effect is still a matter of consideration.
MATERIAL AND METHODSThe materials and methods were unaltered from those described in the preceding paper (Nakamura and Amada, 1958) except for the following points. Washing procedure of red cell: The red cell suspension was centrifcged for 5 minutes at 3,000 rpm at room temperature and the supernatant discarded. Packed cells thus obtained were considered as having been subjected to one washing cycle. Adequate quantities of new saline solution were poured into the tube to make a suspension of original concentration. Microscope photographing o f red cell:The suspension was introduced between a red cell counting chamber and a coverglass, both silicon-coated, and the red cells were photographed by microscope before they sank down all to the bottom.
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