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When a red cell suspension is prepared by suspending un-washed red cells, it may happen that the minute quantities of serum adhering to the cells cause an unexpectedly great change in nature of the suspension and lead to intricacy in studying the interaction between the cells and the artificial medium. By the way, a lasting effect of pretreatment of red cells with saline solution is also observed by several researchers. The irreversible change of washed red cells subjected to its hemolysability in hypo-or hypertonic saline solution, as stated by Kuroda (1936), Ponder (1948) etc., is a case in point.Miyake (1955) also observed the irreversible transformation of non-nucleated erythrocyte, previously washed with the saline solution.In the study on S.L.I.R. of red cell suspension the above effect is still a matter of consideration. MATERIAL AND METHODSThe materials and methods were unaltered from those described in the preceding paper (Nakamura and Amada, 1958) except for the following points. Washing procedure of red cell: The red cell suspension was centrifcged for 5 minutes at 3,000 rpm at room temperature and the supernatant discarded. Packed cells thus obtained were considered as having been subjected to one washing cycle. Adequate quantities of new saline solution were poured into the tube to make a suspension of original concentration. Microscope photographing o f red cell:The suspension was introduced between a red cell counting chamber and a coverglass, both silicon-coated, and the red cells were photographed by microscope before they sank down all to the bottom.