The effects of jump training on bone morphological and mechanical properties were investigated in immature bones of female Fischer 344 rats. Five-week-old rats were divided into control or five jump-trained groups comprised of 5-, 10-, 20-, 40-, and 100-jump groups, representing the number of jumps per day. The rats were jump-trained 5 days/week for 8 weeks, and the height of jump was increased to 40 cm progressively. The femur and tibia in the 5-jump group had significantly greater fat-free dry weights per body weight and maximum loads at the fracture tests than those in the control group. The tibia in the 5-jump group also had significantly larger cortical area at the cross-sectional analysis. Although a slight tendency toward increase according to the number of jumps per day was observed, there were few differences in bone morphological and mechanical parameters among the 10-, 20-, and 40-jump groups. The present results indicate that a large number of strains per day is not necessary for bone hypertrophy to develop in

The effects of jump training on bone hypertrophy were investigated in 3, 6, 12, 20 and 27 month-old female Fischer 344 rats. The rats of all age groups were divided into jump training (height: 40 cm, 100 times/day, 5 days/wk for 8 wk), run training (speed: 30 ml/min, 1 h/day, 5 days/wk for 8 wk) or sedentary group. Fat-free dry weights (FFW) of the femur and the tibia were significantly greater in the jump-trained rats than in the run-trained rats, and were significantly greater in the run-trained rats than in the sedentary rats. Jump training significantly increased FFW of the femur and the tibia not only in young rats but also in old rats, while run training did not increase FFW significantly in old rats. In young rats, both jump training and run training significantly increased the length of the femur and the tibia and the diameter of the femur. The diameter of the tibia was greater in the jump-trained rats than in the sedentary and the run-trained rats in all age groups. The results of the present study indicate that jump training was a more effective training mode than run training for bone hypertrophy and that the effects were not limited by age.

In order to investigate the relationship between metabolic state and myoelectrical activity in working muscle during short term intense exercise, eleven healthy males performed isokinetic knee extensions at an angular velocity of 180 deg X sec-1 for 30 and 60 s. The median frequency (MF) of the surface electromyogram (EMG) recorded from vastus lateralis was decreased while the time lag of torque production after the onset of electrical activity (EMD) was increased during exercise. These changes (MF and EMD) corresponded well to muscle lactate accumulation in the same muscle. Over the exercise period, the integrated EMG/knee extension peak torque ratio (E/T ratio) was increased, which indicated a decrease in the efficiency of electrical activity. It was concluded that the changes in the frequency components of the EMG and in the contractile property of the muscle during short term intense exercise correlated with lactate accumulation in the identical muscle, and that the decrease in efficiency of the electrical activity in the muscle suggested peripheral fatigue.

Keloid is a fibrotic disease characterized by abnormal accumulation of extracellular matrix in the dermis. The keloid matrix contains excess collagen and glycosaminoglycans (GAGs), but lacks elastic fiber. However, the roles of these matrix components in the pathogenesis of keloid are largely unknown. Here, we show that elastin and DANCE (also known as fibulin-5), a protein required for elastic fiber formation, are not deposited in the extracellular matrix of keloids, due to excess accumulation of chondoitin sulfate (CS), although the expression of elastin and DANCE is not affected. Amount of CS accumulated in the keloid legion was 6.9-fold higher than in normal skin. Fibrillin-1, a scaffold protein for elastic fiber assembly, was abnormally distributed in the keloid matrix. Addition of purified CS to keloid fibroblast culture resulted in abnormal deposition of fibrillin-1, concomitant with significantly decreased accumulation of elastin and DANCE in the extracellular matrix. We propose that CS plays a crucial role in the development of keloid lesions through inhibition of elastic fiber assembly.

To investigate the effect of sucrose or caffeine ingestion on the performance of prolonged running, five male distance runners attending senior high school (15.6 yrs) carried out running on a treadmill at an intensity corresponding to the individuals' 80% VO2 max until exhaustion. Before and 45 min after exercise, the subjects were given either a placebo (Con), sucrose (81 +/- 18 g) (Su), caffeine (384 +/- 13 mg) (Caf), or sucrose (72 +/- 22 g) plus caffeine (396 +/- 29 mg) (Su + Caf) solution. The duration of the exercise was significantly longer in Su, Caf, and Su + Caf than in Con. The duration in four of five subjects was longest in Su + Caf, although it was not significantly different from that in Su or Caf. Carbohydrate (CHO) utilization was highest in Su while fat utilization was highest in Caf. The energy supply from both sources was almost the same between Con and Su + Caf. The plasma glucose concentration was higher in Su than in Con. The plasma free fatty acid (FFA) level was higher in Caf than in Con. The plasma glucose and lactic acid concentrations were highest in Su + Caf while the plasma FFA level was the same as in Con. In conclusion, ingestion of sucrose, caffeine, or sucrose plus caffeine solution was equally effective in improving endurance during running carried out at an intensity of approximately 80% VO2 max.

1. The relationship between physical fitness (maximal oxygen uptake VO2max) and incidence of hypertension was investigated through a prospective study for a total of 16,525 human-years of observation. 2. This study involved 3305 Japanese males whose blood pressure (BP) was normal when they received their first physical examination before the age of 50. They were monitored from 1983 to 1988. The BP of 425 subjects was diagnosed as hypertension in the fifth year. 3. Fitness levels were divided into quintiles according to VO2max levels, and were compared with the changes of BP and relative risk of hypertension after adjustment for age, initial percentage of body fat (PFAT), initial BP, alcohol consumption, cigarette smoking status and familial history of hypertension. The increase in BP of subjects in the least fit group was higher than in any other group. Relative risk was calculated using a multiple logistic regression and was 1.9 x higher in the least fit group compared with the fittest group. 4. The subjects were classified into three groups: the improved VO2max group, the deteriorated VO2max group and the unchanged VO2max group. The increase in BP of the improved VO2max group was significantly lower than the other two groups after adjustment for changes in PFAT, age, initial PFAT, initial BP, fitness level, alcohol consumption, cigarette smoking status and familial history of hypertension. 5. It is concluded that low VO2max level is related to higher incidence of hypertension. An improved VO2max would therefore be able to prevent hypertension.

The purpose of this study was to examine the relationships between the physiological characteristics of synchronized swimmers and their performance scores. The subjects were 16 trained female synchronized swimmers with a mean age of 17.2 +/- 1.7 years (mean +/- SD). The examined variables were body dimensions (height, width, body mass, circumference of the body and segment length), body composition, isokinetic muscle strength of the elbow and knee during extension and flexion, abdominal muscle endurance, anaerobic power (leg extension power and peak blood lactate concentration), aerobic power (maximum oxygen uptake [VO2max], swimming velocity at the onset of blood lactate accumulation [OBLA-V]), and flexibility (standing trunk flexion, prone trunk extension and distance between the open legs). The performance scores had significant correlations (p < 0.05) with isokinetic muscle strength of the elbow extension and flexion, and the knee extension, abdominal muscle endurance, leg extension power, VO2max x wt(-1), OBLA-V and distance between the open legs. However, no significant correlations were found between the performance scores and anthropometric variables. This study showed that the performance scores of synchronized swimmers correlated significantly with the functional aspects, and that muscle strength, muscle endurance and aerobic capacity seem to be particularly important determinants.

This study examined the relationship between Olympic distance triathlon (swim over 1.5 km, cycle over 40.0 km, and run over 10.0 km) and economy during a simulated laboratory test triathlon. Seventeen male triathletes conducted both maximal exercise tests and simulated laboratory triathlon. The latter test consisted of swimming (S), cycling (C) and running (R) stages as a continuous task using a flume-pool, a cycle ergometer and a treadmill, respectively. The exercise intensity and duration were 60% of VO2max during swimming, cycling and running for 30, 75 and 45 min, respectively. The index of economy was determined by the % VO2max at the last min of each stage during a simulated laboratory test triathlon. Results indicated that Olympic distance triathlon (total time) was correlated to swimming-VO2max (r = -0.621, p < 0.001), cycling-VO2max (r = -0.873, p < 0.001), running-VO2max (r = -0.891, p < 0.001), S-Economy (r = 0.208, ns), C-Economy (r = 0.601, p < 0.001) and R-Economy (r = 0.769, p < 0.001). There were also significant correlations between swimming time and swimming-VO2max (r = -0.648, p < 0.01), between cycling time and cycling-VO2max (r = -0.819, p <0.001), between running time and running-VO2max (r = -0.726, p < 0.001), between swimming time and S-Economy (r = 0.550, p < 0.05), between cycling time and C-Economy (r = 0.613, p < 0.01), and between running time and R-Economy (r = 0.548, p < 0.05). These results demonstrated that the larger VO2max during maximal exercise tests and smaller increment of VO2 during a simulated laboratory test triathlon indicating good economy were good predictors to determine the Olympic distance triathlon.