Monitoring and optimizing the effectiveness of training course require wide analyses of athletes' blood parameter changes. The aim of this study was to evaluate the usefulness of biochemical liver profile to assess the metabolic response to semi-long-distance outdoor run in football players. Sixteen football players run outdoor for 60 minutes to achieve aerobic metabolism. Plasma activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyltransferase (GGT) and plasma levels of total and direct bilirubin were determined in samples obtained before exercise test (pre-exercise) and immediately after the run (post-exercise). Mean AST plasma activity (U·L-1) before/after the exercise, respectively, was 78.3/228.3 in women and 76.5/56.2 in men. Mean ALT plasma activity (U·L-1) before/after the exercise, respectively, was 27.5/59.1 in women and 36.2/35.3 in men. Mean GGT plasma activity (U·L-1) before/after the exercise, respectively, was 39.3/76.6 in women and 44.7/71.2 in men. Plasma levels of total and direct bilirubin were similar before and after the run regardless of the gender. Statistical significance of the differences between results obtained pre- and post-exercise occurred in women (p = 0.0212 for AST; p = 0.0320 for ALT; p = 0.0067 for GGT, respectively). The training monitoring in athletes should be performed using measurements of performance and biological or physiological parameters. It was found that AST, ALT, and GGT activities could be a valuable tool to assess the metabolic response in high-level fitness female athletes. Therefore, monitoring of those well-known diagnostic markers could prevent the trainee from harmful overtraining.

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It has been suggested that DNA hypomethylation because of poorer effectiveness of the 5,10-methylenetetrahydrofolate reductase (MTHFR) enzyme induces muscular growth. We hypothesised that the common, functional 1298A>C polymorphism in the MTHFR gene is associated with athletic status. To test this hypothesis, we investigated the distribution of the 1298A>C variant in Polish (n = 302) and Russian (n = 842) athletes divided into four groups: endurance, strength-endurance, sprint-strength and strength-endurance, as well as in 1540 control participants. We found different genotypes (the AC heterozygote advantage) and allele distributions among sprint-strength athletes and strength athletes than the groups of sedentary controls for each nationality. In the combined study, the allelic frequencies for the 1298C variant were 35.6% in sprint-strength athletes (OR 1.18 [1.02-1.36], P = 0.024 vs. controls) and 38.6% in strength athletes (OR 1.34 [1.10-1.64], P = 0.003 vs. controls). The results of the initial and repetition studies as well as the combined analysis suggest that the functional 1298A>C polymorphism in the MTHFR gene is associated with athletic status. The presence of the C allele seems to be beneficial in sprint-strength and strength athletes. It needs to be established whether and to what extent this effect is mediated by alteration in DNA methylation status.

To date, no investigation has studied the effect of acute intake of caffeinated chewing gum on volleyball performance. Therefore, the aim of this investigation was to establish the impact of caffeinated chewing gum ingestion on physical performance in female volleyball players. Twelve high-performance volleyball female athletes participated in a randomized, crossover, placebo-controlled, and double-blind experiment. Each athlete performed two identical experimental sessions after a) ingestion of ~6.4 mg/kg of caffeine via caffeinated chewing gum, b) ingestion of non-caffeinated chewing gum as a placebo. After the ingestion of gum, athletes performed a volleyball game, and performance was assessed by a notational analysis. Just before and after the game, jump performance during block and attack actions was evaluated. The number of points obtained and the number of errors committed during serve, reception, attacking, and blocking actions were unaffected by the ingestion of caffeinated chewing gum (p from 0.066 to 0.890). However, caffeinated chewing gum increased jump attack height in comparison to the placebo (pre-game 46.0 ± 7.2 vs. 47.2 ± 6.7 cm, p = 0.032; post-game 46.3 ± 7.6 vs. 47.5 ± 6.9 cm, p = 0.022, respectively). Caffeinated chewing gum did not modify block jump height (pre-game 32.7 ± 5.5 and 33.0 ± 4.3 cm, p = 0.829; post-game: 34.8 ± 6.1, 35.4 ± 6.1 cm, p = 0.993, respectively). The ingestion of ~6.4 mg/kg of caffeine via caffeinated chewing gum was effective for improving jump attack performance in women volleyball athletes. However, this effect was not translated into better volleyball performance during a game.

SummaryBackgroundLong-term and intensive physical effort causes metabolic and biochemical adaptations for both athletic and non-athletic objectives. Knowing the importance of aerobic training in football players, the aim of this study was to evaluate changes in the activity of: creatinine kinase (CK), creatine kinase MB (CKMB), lactate dehydrogenase (LDH), α-hydroxybutyrate dehydrogenase (HBDH), cholinesterase (ChE) and alkaline phosphatase (ALP) in response to a semi-long distance outdoor run under aerobic conditions among both female and male football players.MethodsSixteen participants aged 21.9±2 years (women) and 18.4±0.5 years (men), all of them voluntarily recruited football players, took part in an outdoor run, the women covering a distance of 7.4±0.3 km while men covered a distance of 10.7±1.0 km. Plasma activities of the studied enzymes were determined using an appropriate diagnostic assay kit.ResultsOur results indicate that total LDH activity could be a useful tool in evaluating physical fitness among athletes. We simultaneously established that ChE could not be a marker useful in assessing metabolic response to physical effort in athletes. Moreover, our results suggest that post-effort changes in ALP activity might be used to estimate early symptoms of certain vitamin deficiencies in an athlete’s diet.ConclusionsWe confirmed that the assessment of activity of selected traditional diagnostic enzymatic markers provides information about muscle state after physical effort.