Light-sheet microscopy facilitates rapid, high-contrast, volumetric imaging with minimal sample exposure. However, the rapid divergence of a traditional Gaussian light sheet restricts the field of view (FOV) that provides innate subcellular resolution. We show that the Airy beam innately yields high contrast and resolution up to a tenfold larger FOV. In contrast to the Bessel beam, which also provides an increased FOV, the Airy beam's characteristic asymmetric excitation pattern results in all fluorescence contributing positively to the contrast, enabling a step change for light-sheet microscopy.
Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.
The speckle pattern arising from a thin random, disordered scatterer may be used to detect the transversal mode of an incident beam. On the other hand, speckle patterns originating from meter-long multimode fibers can be used to detect different wavelengths. Combining these approaches, we develop a method that uses a thin random scattering medium to measure the wavelength of a near-infrared laser beam with picometer resolution. The method is based on the application of principal component analysis, which is used for pattern recognition and is applied here to the case of speckle pattern categorization.
A wide array of diffractive structures such as arrays of pinholes, triangular apertures, slits, and holograms have all recently been used to measure the azimuthal index of individual Laguerre-Gaussian beams. Here, we demonstrate a powerful approach to simultaneously measure both the radial and azimuthal indices of pure Laguerre-Gaussian light fields using the method of principal component analysis. We find that the shape of the diffracting element used to measure the mode indices is in fact of little importance and the crucial step is training any diffracting optical system and transforming the observed pattern into uncorrelated variables. The method is generic and may be extended to other families of light fields such as Bessel or Hermite-Gaussian beams.
We demonstrate the first planar Airy light-sheet microscope. Fluorescence light-sheet microscopy has become the method of choice to study large biological samples with cellular or sub-cellular resolution. The propagation-invariant Airy beam enables a tenfold increase in field-of-view with single-photon excitation; however, the characteristic asymmetry of the light-sheet limits its potential for multi-photon excitation. Here we show how a planar light-sheet can be formed from the curved propagation-invariant Airy beam. The resulting symmetric light sheet excites two-photon fluorescence uniformly across an extended field-of-view without the need for deconvolution. We demonstrate the method for rapid two-photon imaging of large volumes of neuronal tissue.
Several phase-modulation functions have been reported to decrease the aberration variance of the modulation-transfer-function (MTF) in aberration-tolerant hybrid imaging systems. The choice of this phase-modulation function is crucial for optimization of the overall system performance. To prevent a significant loss in signal-to-noise ratio, it is common to enforce restorability constraints on the MTF, requiring trade of aberration-tolerance and noise-gain. Instead of optimizing specific MTF characteristics, we directly minimize the expected imaging-error of the joint design. This method is used to compare commonly used phase-modulation functions: the antisymmetric generalized cubic polynomial and fourth-degree rotational symmetric phase-modulation. The analysis shows how optimal imaging performance is obtained using moderate phase-modulation, and more importantly, the relative merits of the above functions.
We demonstrate the transfer of orbital angular momentum to an optically levitated microparticle in vacuum. The microparticle is placed within a Laguerre-Gaussian beam and orbits the annular beam profile with increasing angular velocity as the air drag coefficient is reduced. We explore the particle dynamics as a function of the topological charge of the levitating beam. Our results reveal that there is a fundamental limit to the OAM that may be transferred to a trapped particle, dependent upon the beam parameters and inertial forces present.
Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an “optimal” light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.
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