Purpose: The present study was undertaken to develop a validated, rapid, simple and low-cost ultraviolet (UV) spectrophotometric method for estimating levofloxacin (LFX) in dosage preparations. Method: UV spectrophotometric analysis was performed spectrophotometrically at a pre-determined λmax of 290 nm with 0.1M HCl as diluent/blank. The method was validated for linearity, accuracy, precision, reproducibility, and specificity as per International Conference on Harmonization (ICH) guidelines. The method was also used in the determination of the content of levofloxacin in two commercial brands of levofloxacin in the Nigerian market. Results: The regression data for the calibration plots exhibited good linear relationship (r = 0.999) over a concentration range of 0.25 -12.0 µg/ml and the linear regression equation was y = 0.075x + 0.018. Mean recovery accuracy was 98.7 %, which was not significantly different from the expected value (p = 0.05), while coefficient of variation (CV) for both intra-day and inter-day was < 7 %. The method was specific for levofloxacin in the presence of common excipients, and when it was applied to two marketed brands, levofloxacin content was 99. 69 ± 2.38 and 102.65 ± 3.64 %, respectively, of labeled claim. Conclusion: The proposed method gave good validation results and the statistical analysis performed proved that the method is precise, accurate and reproducible, and hence can be employed for routine analysis of LFX in bulk and commercial formulations.
The increasing resistance profile of many bacterial and yeast strains to conventional antimicrobial agents has heightened the search for bioactive compounds from higher plants that would serve as lead molecules in the discovery and development of new drugs. This study determined the antimicrobial properties and chemical constituents of the ethyl acetate extract of Annona muricata seeds. Crude extract obtained by maceration of pulverized seeds of Annona muricata in ethyl acetate was evaluated for antimicrobial activity against six clinical bacterial isolates (Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Klebsiella aerogenes) and one fungus (Candida albicans) using agar-well diffusion method. GC-MS method was used to determine the chemical constituents of the extract. The extraction yielded 19.93% of an oily yellow crude mass. The extract inhibited the growth of all test bacteria with inhibitory zone diameters range of 23.5 - 17.02 mm and 25.90 - 31.50 mm for the standard antimicrobial agents. The GC-MS analysis identified 48 chemical constituents in the extract with 9,12-octadecadienal, 1,14-tetradecadienal, phthalic and fatty acids accounting for 15.26, 7.77, 2.53 and 11.20% respectively. The data from this study revealed the possible antimicrobial potentials and chemical constituents of Annona muricata seeds and hence the scientific basis for its traditional use in the treatment of some infections.
(-5.78 mol.kg-1) and constant (-1141 mol-1L
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.