Soy sauce secondary precipitate formed due to the B 3 subunit seriously affects soy sauce's appearance quality. In this study, a prolyl endopeptidase (APE) from Aspergillus niger, which could degrade approximately 50% of the B 3 subunit and increase proline content by 24% in soy sauce, was isolated and identified. The results showed that APE was an acidic salt-tolerant serine protease (62 kDa), which was optimally active at 40 °C and pH 4.0, and retained more than 69% activity in 3 M NaCl solution over 10 days. As a potential substrate of APE, the B 3 subunit contains 10 proline residues. High salinity could not damage the hydrogen bonds, salt bridges, and interior hydrophobicity of APE; thus, the spatial structures and activity of APE in 3 M NaCl solution were stable within 3 days and decreased thereafter. High salinity made the B 3 subunit more rigid and lowered the catalytic activity of APE on the B 3 subunit, hindering complete hydrolysis of the B 3 subunit. This was the first report about the APE capable of degrading the B 3 subunit and reducing the secondary precipitate of soy sauce, providing a new possibility to solve the secondary precipitate of soy sauce.
Ginkgo biloba seed has antimicrobial activity. In this study, ginkgo biloba seed protein was prepared, identified, and named GBSPI-A, finding its construction was similar to 11-S globulin. Then, the influence of GBSPI-A on the cell membrane and physiological metabolism of K. pneumoniae and S. aureus were investigated. The results showed that GBSPI-A (20 mg/mL) destroyed the cell membrane, causing leakage of intracellular material and inhibited bacterial growth with an inhibition rate of approximately 80%. In addition, the GBSPI-A (10 mg/mL) caused the decreasing activity of ATPase and respiratory rate, and the respiratory depression rate was 7.24%. Furthermore, the decreasing ATP synthesis and intracellular β-galactosidase activity led to an insufficient supply of physiological metabolic energy. Therefore, the results showed that GBSPI-A could be used as a natural bacteriostatic agent to replace related drugs and also provide a new insight into the application of GBSPI-A in food safety.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.