Combining
biocatalytic and chemocatalytic reactions in a one-pot
reaction not only avoids the tedious isolation of intermediates during
the reactions but also provides a desirable alternative to extend
the range of catalytic reactions. Here, we report a facile strategy
to immobilize an enzyme, glucose oxidase (GOx), on PCN-222(Fe) induced
by electrostatic interaction in which PCN-222(Fe) serves as both a
support and chemocatalyst. The immobilization was confirmed through
ζ potential measurement, confocal laser scanning microscopy,
Fourier transform infrared spectrometry, and UV–vis spectroscopy.
This chemo-biocatalyst was applied to a cascade reaction to catalyze
glucose oxidation and ABTS (ABTS = 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic
acid) (or pyrogallol) oxidation. The catalytic kinetics studies show
that these chemo-biocatalytic cascade reactions obey the Michaelis–Menten
equation, which indicates that the cascade reactions follow the typical
enzymatic dynamic regulation process. Interestingly, GOx/PCN-222(Fe)
exhibits an exceptional acid-stable catalytic performance as evidenced
by circular dichroism spectroscopy where no significant structure
change was observed toward acidic solutions with different pH values.
GOx/PCN-222(Fe) also displays desirable recyclability since no significant
loss of conversion rates was found after six repeated reactions. This
work presents a convenient strategy to construct metal–organic
framework based chemo-biocatalysts, which may find potential applications
in sensing and nanomachines.
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