Polyploidy, a state in which the chromosome complement has undergone an increase, is a major force in evolution. Understanding the consequences of polyploidy has received much attention, and allopolyploids, which result from the union of two different parental genomes, are of particular interest because they must overcome a suite of biological responses to this merger, known as “genome shock.” A key question is what happens to gene expression of the two gene copies following allopolyploidization, but until recently the tools to answer this question on a genome-wide basis were lacking. Here we utilize high throughput transcriptome sequencing to produce the first genome-wide picture of gene expression response to allopolyploidy in fungi. A novel pipeline for assigning sequence reads to the gene copies was used to quantify their expression in a fungal allopolyploid. We find that the transcriptional response to allopolyploidy is predominantly conservative: both copies of most genes are retained; over half the genes inherit parental gene expression patterns; and parental differential expression is often lost in the allopolyploid. Strikingly, the patterns of gene expression change are highly concordant with the genome-wide expression results of a cotton allopolyploid. The very different nature of these two allopolyploids implies a conserved, eukaryote-wide transcriptional response to genome merger. We provide evidence that the transcriptional responses we observe are mostly driven by intrinsic differences between the regulatory systems in the parent species, and from this propose a mechanistic model in which the cross-kingdom conservation in transcriptional response reflects conservation of the mutational processes underlying eukaryotic gene regulatory evolution. This work provides a platform to develop a universal understanding of gene expression response to allopolyploidy and suggests that allopolyploids are an exceptional system to investigate gene regulatory changes that have evolved in the parental species prior to allopolyploidization.

Abstract:Although structural health monitoring (SHM) technologies using sensors have dramatically been developed recently, their capability should be evaluated from the perspective of the maintenance industry. As a first step toward utilizing sensors, the objective of the paper is to investigate the possibility of using sensors for inspecting the entire fuselage during C-check. First, we reviewed various sensors for their detection range, detectable damage size, and installed weight, which revealed that the piezoelectric wafer active sensor (PWAS) is the most promising sensor for aircraft SHM. Second, we performed a case study of inspecting the fuselage of Boeing-737NG using PWAS. To maintain the same detecting capability of manual inspection in C-check, we estimated the total number of sensors required. It turned out that utilizing sensors can reduce the maintenance downtime and thus, maintenance cost. However, even with a very conservative estimate, the lifetime cost was significantly increased due to the weight of sensor systems. The cost due to the weight increase was an order of magnitude higher than the cost saved by using SHM. We found that a large number of sensors were required to detect damage at unknown locations, which was the main cause of the weight increase. We concluded that to make SHM cost-effective, it would be necessary either to improve the current sensor technologies so that a less number of sensors are used or to modify the aircraft design concept for SHM.

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Recently, thermally activated delayed fluorescence (TADF) organic light-emitting diodes (OLEDs) have attracted both academic and industrial interest due to their extraordinary characteristics, such as high efficiency, low driving voltage, bright luminance, lower power consumption and potentially long lifetime. In this invited review, the fundamental concepts of TADF have been firstly introduced. Then, main approaches to realize WOLEDs based on TADF have been summarized. More specifically, the recent development of WOLEDs based on all TADF emitters, WOLEDs based on TADF and conventional fluorescence emitters, hybrid WOLEDs based on blue TADF and phosphorescence emitters and WOLEDs based on TADF exciplex host and phosphorescence dopants is highlighted. In particular, design strategies, device structures, working mechanisms and electroluminescent processes of the representative WOLEDs based on TADF are reviewed. Finally, challenges and opportunities for further enhancement of the performance of WOLEDs based on TADF are presented.