Personal protective equipment (PPE) can potentiate heat stress which may negatively impact wearer’s performance, safety and well-being. In view of this, a survey was distributed to healthcare workers (HCWs) required to wear PPE during the COVID-19 pandemic in the UK to evaluate perceived levels of heat stress and its consequences. Respondents reported experiencing several heat-related illness symptoms and that heat stress impaired both cognitive and physical performance. The majority also reported PPE made their job more difficult. These, and additional responses, suggest that modification to current working practices is urgently required to improve HCWs’ resilience to wearing PPE during pandemics.

Enabling successful active ageing is an international priority to meet the challenges of increasing life expectancy. Digital strategies, such as telemedicine and e-health, offer the potential to deliver active ageing in a cost-effective manner at scale. This article aims to establish the extent to which the research literature considers e-health-based and telemedicine-based active ageing interventions. A systematic review was conducted according to PRISMA standards. Independently, two authors searched the Cochrane, EMBASE & CINAHL databases, with subsequent independent extraction and semi-quantitative analysis. We report a considerable breadth in digital active ageing research, which is truly international in its scope. There is a diverse range of both interventions and technologies, including a reassuring focus on community-based interventions. Whilst there are a number of quantitative studies, sample sizes are small, with a limited amount of statistical testing of the results. There is significant variation in the outcome measures reported and little consensus as to the most effective intervention strategies. Overall, whilst there is considerable breadth to the research published in the literature, there is a clear restriction in the depth of this research. There is little overall consensus. This lack of depth and consensus may be due to the need to recognize the important role of technical research elements alongside more traditional research methodologies, such as randomized controlled trials. Enabling both technical and clinical research methods to be recognized, in tandem, has enormous potential to support individuals, communities, clinicians and policy makers to make more informed decisions in relation to active ageing.

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Werner syndrome (WS) is a rare late-onset premature ageing disease showing many of the phenotypes associated with normal ageing, and provides one of the best models for investigating cellular pathways that lead to normal ageing. WS is caused by mutation of WRN, which encodes a multifunctional DNA replication and repair helicase/exonuclease. To investigate the role of WRN protein’s unique exonuclease domain, we have recently identified DmWRNexo, the fly orthologue of the exonuclease domain of human WRN. Here, we fully characterise DmWRNexo exonuclease activity in vitro, confirming 3′–5′ polarity, demonstrating a requirement for Mg2+, inhibition by ATP, and an ability to degrade both single-stranded DNA and duplex DNA substrates with 3′ or 5′ overhangs, or bubble structures, but with no activity on blunt ended DNA duplexes. We report a novel active site mutation that ablates enzyme activity. Lesional substrates containing uracil are partially cleaved by DmWRNexo, but the enzyme pauses on such substrates and is inhibited by abasic sites. These strong biochemical similarities to human WRN suggest that Drosophila can provide a valuable experimental system for analysing the importance of WRN exonuclease in cell and organismal ageing.Electronic supplementary materialThe online version of this article (doi:10.1007/s11357-012-9411-0) contains supplementary material, which is available to authorized users.

Cardiopulmonary exercise testing (CPET) allows objective assessment of a patient’s global response to maximal incremental exercise. CPET has been proposed to have a role in investigating post-COVID syndrome. However, CPET is resource intensive, and essential for restoration of other clinical services (e.g. cancer surgery). The aim of this study was to explore utility of CPET in assessing functional status of COVID-19 survivors with persistent dyspnoea. Of the 600 patients reviewed in a post-COVID-19 assessment clinic between May 2020 and April 2021, 12 (male/female: 8/4; age: 4±15.2 years; BMI: 32.8±5.9 kg/m2; non-smokers/ smokers: 8/4) were referred for CPET due to persistent breathlessness out-keeping with disease severity. Of these patients, 10 patients demonstrated reduced peak VO2, whilst five had an exercise limitation attributed to physical deconditioning. Two patients had mainly a cardiac limitation to exercise, with a further three patients demonstrating breathing pattern disorder, pulmonary vascular disease and lung disease. The findings of this single-centre study suggest that intensive CPET testing may not add substantial additional clinical information to aid patient investigation/management in the context of post-COVID. Such resource intensive procedures may be better utilised in selected patients and in the restoration of NHS services following the COVID-19 pandemic.

Non-Hodgkin lymphomas of the hypothalamus and pituitary are rare. They usually remain clinically silent until onset of compressive features affecting surrounding structures. When symptomatic, patients most commonly present with diabetes insipidus, headaches, ophthalmoplegia and/or bilateral hemianopia.  We report a case of a 67-year-old Caucasian female with a history of B-cell lymphoma in complete remission. She presented with left oculomotor nerve palsy and was subsequently found to have a sellar/suprasellar mass lesion on MRI. Alongside hypocortisolism and hypogonadotropic hypogonadism, she developed transient diabetes insipidus during her illness. Her clinical course was characterized by rapid intracranial progression of the sellar mass. MR spectroscopy suggested a diagnosis of lymphoma. Diagnostic biopsy confirmed high-grade diffuse large B-cell CNS lymphoma; this changed the definitive management from surgical excision to chemotherapy. Despite treatment, she succumbed to her illness within 7 months of initial presentation. This case highlights the aggressive nature of CNS lymphomas and the need for a high index of suspicion in an unusual presentation of sellar/suprasellar mass lesions.

Background: The impermeable nature of PPE worn by health care workers (HCWs) during the SARS-CoV-2 (COVID-19) pandemic can potentiate heat stress which may negatively impact the performance, safety and well-being of HCWs. Aim: The aim of this study was to evaluate perceived levels of heat stress and its consequences in HCWs required to wear PPE during the COVID-19 pandemic in the UK. Method: An anonymous online survey was distributed to HCWs required to wear Type 1 or 2 PPE in NHS settings to evaluate the perceived impact of PPE on: (1) physical and cognitive performance; (2) heat stress and heat-related symptoms; (3) frequency of removing PPE due to discomfort caused by heat stress; and (4) general working-life and well-being. Results: The survey received 224 responses from 192 (85.7%) women and 32 (14.3%) men. Even though 71.9% of respondents wore the less thermally challenging PPE (i.e. Type 2), a median of 3 (IQR: 2,5) heat-related symptoms were reported including syncope (7.7%). A median of 1 (IQR: 0-3) cognitive task was adversely affected with attentional focus being the most affected. To relieve discomfort, 32.6% reported removing PPE on five or more occasions in a shift. Ninety two percent reported PPE made their job more difficult and 76.2% advised that physical performance was impaired. Respondents also highlighted concerns of dermatitis and pressure sores in the facial region (22.3%) amongst other factors. Conclusion: Heat stress experienced when PPE is worn negatively impacts the performance, safety and well-being of HCWs and patients. Therefore, modification to current working practices and current design of PPE is urgently required to improve HCWs resilience to pandemics of infectious diseases. Results suggest modifications to the design of the protective face mask and strict enforcement of specific work/rest ratios to limit the duration of PPE use would be immediate impactful interventions.

G training programme director for diabetes and endocrinology, Health Education West Midlands, Birmingham, UK and consultant in diabetes and endocrinology, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK ResultsUsing digital technologies reduced both costs and administrative burdens. Simulation-based learning helped improve trainees' self-reported confidence levels. ConclusionCollaborative working across training programme directors, specialist training committee members, educational supervisors, trainee representatives and trainees themselves can develop high-quality educational programmes that support clinical exposure. We propose that elements of the model described here can be replicated across regions and different specialties to support the highest quality of education for UK trainees.