Time-resolved fluorescence detection for robust sensing of biomolecular interactions is developed by implementing time-correlated single photon counting in high-throughput conditions. Droplet microfluidics is used as a promising platform for the very fast handling of low-volume samples. We illustrate the potential of this very sensitive and cost-effective technology in the context of an enzymatic activity assay based on fluorescently-labeled biomolecules. Fluorescence lifetime detection by time-correlated single photon counting is shown to enable reliable discrimination between positive and negative control samples at a throughput as high as several hundred samples per second.
This paper presents a Fixed Priority Arbiter (FPA) which fully takes benefice of asynchronous design. It signs up in a Time Correlated Single Photon Counting system where the data transfer from the Time to Digital Converters to the readout or process unit is a critical aspect. In order to increase the photon counting rate of the setup, the device has to handle a large amount of data following the increasing number of sensors. Thanks to its asynchronous design, the proposed FPA can be easily assembled to build a wide data path tree to guide all the data to a single output. The implementation on a 180 nm CMOS technology shows that a 128 input wide path tree can achieve a burst input rate of 21 Giga event per second while sustaining an output data rate of 333 Mega event per second.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.