Background/Aims: Cancer stem cells (CSCs) exhibit enhanced proliferative capacity and resistance to chemotherapy; however, choriocarcinoma CSCs have not yet been reported. In this study the human choriocarcinoma cell line JEG-3 was cultured in serum free media, and the characteristics of suspension and parental adherent JEG-3 cells were compared. Methods: Cell proliferation, colony-formation, soft agar clonogenicity, and transwell invasion assays were performed in vitro, and tumor xenografts in BALB/c nude mice were used to evaluate stem cell properties. Results: In serum-supplemented medium (SSM), JEG-3 cells were 4.51 ± 1.71% CD44+, 7.67 ± 2.67% CD133+, and 13.85 ± 2.95% ABCG2+. In serum-free medium (SFM), the expression of these markers increased to 53.08 ± 3.15%, 47.40 ± 2.67%, and 78.70 ± 7.16%, respectively. Moreover, suspension JEG-3 cells exhibited enhanced colony-formation capability as well as invasive and proliferative ability in vitro, alongside enhanced tumorigenic properties in vivo. Suspension JEG-3 cells also exhibited resistance to the chemotherapeutic drugs methotrexate, fluorouracil and etoposide. When seeded in serum supplemented medium, suspension JEG-3 cells readopted an adherent phenotype and continued to differentiate with no significant difference in the morphology between suspension and parent cells. Conclusion: In this study, choriocarcinoma stem-like cells (CSLCs) were isolated from the human choriocarcinoma JEG-3 cell line by SFM culture and characterized.
The activity of pyrite
(FeS2) and microsized zero-valent
iron (mZVI) is greatly limited by the passivating layer. This study
indicated that hexavalent chromium (Cr(VI)) could be effectively sequestered
by mechanochemically activated mZVI/FeS2 composite (MA-mZVI/FeS2), which was obtained from the mZVI/FeS2 mixture
by mechanochemical technology. Compared with MA-mZVI and MA-FeS2, the Cr(VI) removal performance of MA-mZVI/FeS2 was significantly improved at a mZVI/FeS2 molar ratio
of 1:9. The effects of initial concentration, coexisting anions, reaction
time, and initial pH on Cr(VI) sequestration by MA-mZVI/FeS2 were investigated. During the removal process, the concentrations
of different valence states of chromium and iron were measured to
explore the Cr(VI) removal mechanism. Based on the experiments and
characterizations, surface sorption accounted for 41.38% and reduction
accounted for 58.62% at the Cr(VI) adsorption equilibrium of MA-mZVI/FeS2. The ball milling destroyed the passivating layer of MA-mZVI/FeS2, resulting in a rough surface. The corrosion of mZVI can
be significantly accelerated by FeS2 due to the strong
interaction between mZVI and FeS2 in MA-mZVI/FeS2, which led to the enhanced Cr(VI) sequestration. The practical application
indicated that MA-mZVI/FeS2 had good Cr(VI) removal performance
in real water samples. This study revealed that MA-mZVI/FeS2 can be a hopeful adsorbent for effective Cr(VI) sequestration in
aqueous solution.
LncRNA ATB correlates with the malignant phenotypes and poor prognosis of cervical cancer. ATB may serve as a promising prognostic marker and therapeutic target of cervical cancer patients.
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