X-chromosomal STRs (X-STRs) have been used as complements of autosomal STR application in recent years. In this work, we present population genetic data of 12 X-STRs including DXS101, DXS10159, DXS10162, DXS10164, DXS6789, DXS7133, DXS7423, DXS7424, DXS8378, DXS981, GATA165B12, and GATA31E08 loci in a sample of 231 unrelated healthy individuals from the Hui ethnic group in Ningxia Hui Autonomous Region, China. Allelic frequencies of the 12 X-STR loci and haplotypic frequencies of the reported linkage groups (DXS7424-DXS101 and DXS10159-DXS10164-DXS10162) were investigated in the group, respectively. No STR loci showed significant deviations from the Hardy-Weinberg equilibriums and no linkage disequilibriums of pairwise loci were found after Bonferroni correction, respectively. A combined power of discrimination in female individuals was 0.999999999985 and that in male individuals was 0.99999967, respectively. The combined mean exclusion chance in deficiency cases, normal trios and duo cases were 0.999934, 0.995754, and 0.999796, respectively. Significant differences were observed from 0 to 8 loci, when making comparisons between the data of Hui ethnic group and previously reported data from other 16 populations. The results indicated the new panel of 12 X-STR loci might be useful for forensic science application.
Availability evaluation of a virtualized system is critical to the wide deployment of cloud computing services. Time-based, prediction-based rejuvenation of virtual machines (VM) and virtual machine monitors (VMM), VM failover, and live VM migration are common high-availability (HA) techniques in a virtualized system. This paper investigates the effect of combination of these availability techniques on VM availability in a virtualized system where various software and hardware failures may occur. For each combination, we construct analytic models rejuvenation mechanisms improve VM availability; (2) prediction-based rejuvenation enhances VM availability much more than time-based VM rejuvenation when prediction successful probability is above 70%, regardless failover and/or live VM migration are also deployed; (3) failover mechanism outperforms live VM migration, although they can work together for higher availability of VM. In addition, they can combine with software rejuvenation mechanisms for even higher availability; (4) and time interval setting is critical to a time-based rejuvenation mechanism. These analytic results provide guidelines for deploying and parameter setting of HA techniques in a virtualized system.
Background
Wild sugarcane
Saccharum spontaneum
plants vary in ploidy, which complicates the utilization of its germplasm in sugarcane breeding. Investigations on cold tolerance in relation to different ploidies in
S. spontaneum
may promote the exploitation of its germplasm and accelerate the improvement of sugarcane varieties.
Results
A hypoploid clone 12–23 (2n = 54) and hyperploid clone 15–28 (2n = 92) of
S. spontaneum
were analysed under cold stress from morphological, physiological, and transcriptomic perspectives. Compared with clone 15–28, clone 12–23 plants had lower plant height, leaf length, internode length, stem diameter, and leaf width; depressed stomata and prominent bristles and papillae; and thick leaves with higher bulliform cell groups and thicker adaxial epidermis. Compared with clone 15–28, clone 12–23 showed significantly lower electrical conductivity, significantly higher water content, soluble protein content, and superoxide dismutase activity, and significantly higher soluble sugar content and peroxidase activity. Under cold stress, the number of upregulated genes and downregulated genes of clone 12–23 was higher than clone 15–28, and many stress response genes and pathways were affected and enriched to varying degrees, particularly sugar and starch metabolic pathways and plant hormone signalling pathways. Under cold stress, the activity of 6-phosphate glucose trehalose synthase, trehalose phosphate phosphatase, and brassinosteroid-signalling kinase and the content of trehalose and brassinosteroids of clone 12–23 increased.
Conclusions
Compared with hyperploid clone 15–28, hypoploid clone 12–23 maintained a more robust osmotic adjustment system through sugar accumulation and hormonal regulation, which resulted in stronger cold tolerance.
Movements of the western Pacific subtropical high (WPSH) are an important factor dominating the synoptic weather and climate over East Asia. In this study, characteristics of the WPSH meridional movement are statistically analysed for the tropical cyclone (TC) peak months (July-September) from 2001 to 2010. The results show that the WPSH meridional movement is consistent overall, namely, its ridge line moves synchronously with its main body centroid, with a correlation coefficient of 0.70 between the ridge line and main body centroid. Moreover, the sensitivity experiment with the Weather Research and Forecasting Model (WRF) for super typhoon Megi (2010) demonstrates that TCs can affect the WPSH meridional movement by stimulating abnormal perturbations that disperse and propagate outwards. Specifically, the WPSH ridge line would shift northwards at an average of 0.64 latitude and a maximum of 1.44 latitude for the period when TC Megi moves to the south of the WPSH. In summary, the meridional movement of the WPSH ridge and centroid are well-correlated and are strongly influenced by TC activity. This study proposes a possible way by which TCs over western North Pacific could affect the weather and climate in East Asia.
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