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IntroductionFlaxseed Linum usitatissimum L. is a potential oilseed crop and widely planted in China, North America, Ethiopia and India. Flaxseed contains 30-40 of oil 1 . It has been reported that daily intake of flaxseed oil can reduce the risk of colon tumors, mammary cancer, atherosclerosis, etc. 2 . The efficacy of flaxseed oil is mainly attributed to the richness in unsaturated fatty acids, especially α-linolenic acid ALA 18:3, n-3 , which accounts for 40-68 3 . The consumption of flaxseed oil helps to balance the proportion of n-3 and n-6 fatty acids in the human diet 4 . Flaxseed oil has been considered a healthy oil for its nutritional and pharmaceutical value.Mechanical pressing and organic solvent extraction are the two most common methods for vegetable oil production. However, organic solvent extraction is unacceptable on account of the inevitable residual solvent that is harmful to human health 5 . While mechanical pressing is high energy consumption and low extraction rate 6 . Supercritical CO 2 extraction seems to be an innovative and acceptable method because it is non-toxic, organic solvents-free, and allows more selectivity for target production. However, it is suffering from some serious problems such as high operating pressure, high cost of instrument, and limited production capacity 7 . Compared with supercritical CO 2 extraction, subcritical fluid extraction has advantages of mild

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