This
paper proposes a simple and robust method for spontaneously
digitizing aqueous samples into a high-density microwell array. The
method is based on an oil-triggered template-confined dewetting phenomenon.
To realize the dewetting-induced sample digitization, an aqueous sample
is first infused into a networked microwell array (NMA) through a
pre-degassing-based self-pumping mechanism, and an immiscible oil
phase is then applied over the surface of NMA chip to induce the templated
dewetting. Due to periodic interfacial tension heterogeneity, such
dewetting ruptures the sample at the thinnest parts (i.e., connection channels) and spontaneously splits the sample into
droplets in individual microwells. Without requiring any complex pumping
or valving systems, this method can discretize a sample into tens
of thousands of addressable droplets in a matter of minutes with nearly
98% usage. To demonstrate the utility and universality of this self-digitization
method, we exploited it to discretize samples into 40 233 wells
for a digital PCR assay, the digital quantification of bacteria, the
self-assembly of spherical colloidal photonic crystals, and the spherical
crystallization of drugs. We believe this facile technique will provide
a substantial benefit to many compartmentalized assays or syntheses
where it is necessary to partition samples into a large number of
small individual volumes.
Digital bioassays are powerful methods to detect rare analytes from complex mixtures and study the temporal processes of individual entities within biological systems. In digital bioassays, a crucial first step is the discretization of samples into a large number of identical independent partitions. Here, we developed a rapid and facile sample partitioning method for versatile digital bioassays. This method is based on a detachable self-digitization (DSD) chip which couples a reversible assembly configuration and a predegassing-based self-pumping mechanism to achieve an easy, fast and large-scale sample partitioning. The DSD chip consists of a channel layer used for loading sample and a microwell layer used for holding the sample partitions. Benefitting from its detachability, the chip avoids a lengthy oil flushing process used to remove the excess sample in loading channels and can compartmentalize a sample into more than 100,000 wells of picoliter volume with densities up to 14,000 wells/cm2 in less than 30 s. We also demonstrated the utility of the proposed method by applying it to digital PCR and digital microbial assays.
In this work, we present a simple, straightforward, and robust method for spontaneously digitizing samples into an array of dis-crete volumes. The method is based on an oil-triggered, template-confined dewetting phenomenon. To realize the dewetting-induced sample digitization, an aqueous sample is firstly infused into a networked microwell array (NMA) through a predegassing-based self-pumping mechanism, and then an immiscible oil phase is applied over the surface of NMA chip to induce the templated dewetting. Due to a periodic interfacial tension heterogeneity, such dewetting ruptures the sample at the thinnest parts (i.e., con-nection channels) and spontaneously splits the sample into droplets in individual microwells. Without requiring any complex pump-ing or valving systems, this method can discretize a sample into tens of thousands of addressable droplets in a matter of minutes with nearly 98% usage. To demonstrate the utility and universality of this self-digitization method, we exploited it to discretize samples into 40,233 wells for digital PCR assay, digital quantification of bacteria, and self-assembly of spherical colloidal photonic crystals. We believe this facile technique will be useful in a broad range of applications where partitioning of samples into a large number of small individual volumes is required.
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