, T. 2001. Impacts of clearcut harvesting and wildfire on soil nutrient status in the Quebec boreal forest. Can. J. Soil Sci. 81: 229-237. Wildfire has historically been the major cause of stand initiation in the boreal forest, shaping species diversity, successional and ecosystem processes. Clearcut harvesting may differ from fire in its effects on soil and vegetation processes and thus may cause long-term changes in stand productivity or biodiversity. This study compared the soil properties of mesic black spruce (Picea mariana) stands burned 2, 14, 21 yr prior to sampling, with stands clearcut within ±3 yr of each wildfire and recently undisturbed control stands. The forest floor (FH) and mineral soil (0-10 cm) were sampled volumetrically, air dried and analysed for pH, organic carbon content, available P, Ca, Mg, and K, mineralizable N and nitrification. Forest floors were also digested and analysed for total N, P, K, Ca and Mg. Significant differences between disturbed and control stands were observed in all study areas, with disturbance effects generally decreasing with time since disturbance. Burned stands generally had forest floors with thinner humus layers, lower mass of organic carbon, higher pH, and higher concentrations of total and available nutrients than in either clearcut or control stands. Significant losses in the total mass of N and K in the forest floor were observed in the youngest burned stands as well as a pulse of extractable P that was at least four times higher than cut or control stands in any other treatment or study area. The forest floor of cut stands had greater mass of organic matter and total nutrients, and higher levels of potentially mineralizable N than either fire or control stands. No significant nutrient loss was observed following clearcut harvesting in any study area. Overall, this study suggested that clearcut harvesting can result in changes to the status of soil nutrients that are different from those produced by wildfire. Further study is necessary to determine whether these differences have significant effects on the long-term productivity or biodiversity of the boreal forest. Les feux irréprimés ont toujours été la principale cause du renouvellement des peuplements dans la forêt boréale. Ce sont eux qui commandent la diversité des espèces, la succession de ces dernières et les fonctionnement de l'écosystème. La coupe à blanc pourrait ne pas avoir les mêmes conséquences sur les processus du sol et de la végétation, et on pourrait se assister à des changements à long terme au niveau de la productivité ou de la diversité biologique du peuplement. Les auteurs ont comparé les propriétés du sol de peuplements mésiques d'épinettes noires (Picea mariana) ravagés par le feu 2, 14 et 21 ans avant l'échantillonnage à celles du sol de peuplements coupés à blanc plus ou moins trois ans avant les feux de forêt et de peuplements témoins intacts. Les auteurs ont prélevé des échantillons volumétriques de la couverture morte (CM) et du sol minéral (de 0 à 10 cm), les ont séchés à l'a...

Legionella pneumophila is a Gram-negative bacterium that replicates within human alveolar macrophages by evasion of the host endocytic pathway through the formation of a replicative vacuole. Generation of this vacuole is dependent upon the secretion of over 275 effector proteins into the host cell via the Dot/Icm type IVB secretion system (T4SS). The type IV coupling protein (T4CP) subcomplex, consisting of DotL, DotM, DotN, IcmS and IcmW, was recently defined. DotL is proposed to be the T4CP of the L. pneumophila T4SS based on its homology to known T4CPs, which function as inner-membrane receptors for substrates. As a result, DotL is hypothesized to play an integral role(s) in the L. pneumophila T4SS for the engagement and translocation of substrates. To elucidate this role, a genetic approach was taken to screen for dotL mutants that were unable to survive inside host cells. One mutant, dotLY725Stop, did not interact with the type IV adaptor proteins IcmS/IcmW (IcmSW) leading to the identification of an IcmSW-binding domain on DotL. Interestingly, the dotLY725Stop mutant was competent for export of one class of secreted effectors, the IcmSW-independent substrates, but exhibited a specific defect in secretion of IcmSW-dependent substrates. This differential secretion illustrates that DotL requires a direct interaction with the type IV adaptor proteins for the secretion of a major class of substrates. Thus, by identifying a new target for IcmSW, we have discovered that the type IV adaptors perform an additional role in the export of substrates by the L. pneumophila Dot/Icm T4SS.

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MicroRNAs (miRNAs) are small RNAs that regulate gene expression. miRNAs are produced from primary miRNAs (pri-miRNAs), which are cleaved by Microprocessor. Microprocessor, therefore, plays a crucial role in determining the efficiency and precision of miRNA production, and thus the function of the final miRNA product. Here, we conducted highthroughput enzymatic assays to investigate the catalytic mechanism of Microprocessor cleaving randomized pri-miRNAs. We identified multiple mismatches and wobble base pairs in the upper stem of pri-miRNAs, which influence the efficiency and accuracy of their processing. The existence of these RNA elements helps to explain the alternative cleavage of Microprocessor for some human pri-miRNAs. We also demonstrated that miRNA biogenesis can be altered via modification of the RNA elements by RNA-editing events or single nucleotide polymorphisms (SNPs). These findings improve our understanding of pri-miRNA processing mechanisms and provide a foundation for interpreting differential miRNA expression due to RNA modifications and SNPs.