No abstract

Pyran copolymer, a known immunostimu- Single-stranded homopolymers of ribonucleotides are reported to be inhibitory to viral RNA-dependent DNA polymerase (6, 7). This observation suggests to us that it may be possible to design molecules that will possess some relationship to the template of the reaction.The active compounds studied are pyran copolymers, the structure of which is shown in Scheme 1. It is a copolymer of approximate molecular weight of 17,000. It is a divinyl ether of maleic anhydride which, in the presence of H20, becomes hydrolyzed to the corresponding polyearboxylic acids. The compound is stable to chemical and enzymatic hydrolysis; it cannot serve as a template in the polymerase reaction.The highly negative charges mimic the negative charges of the nucleotide templates.In this report we describe the mechanism of action of pyran on the purified RNA-dependent DNA

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Immunodifusion testing of cellular antigen extracts from herpes-type virus (HTV, EB V ) -infected and -uninfected

The development of a sensitive immunodiffusion test for precipitating antibodies to antigens derived from herpes-type virus (HTV) infected Burkitt lymphoma cells in tissue culture is described. Seroepidemiologic observations with 517 sera were made. Precipitating antibody was found in a majority of the normal adult population, in contrast to some previous reports. The number of precipitin bands obtained was directly related to the titer of antibody reacting in indirect immunofluorescence. Antibody activity appears markedly increased in the sera of patients with Burkitt’s lymphoma, as shown by percent positive and number of precipitin bands. Studies of the antigens suggest that the precipitins are virus-specific, and may be virion protein antigens.