Petroleum ether extracts of 63 varieties of plant species, including 37 varieties of leaves, 14 varieties of seeds, 10 varieties of flowers and 2 varieties of fruits were tested for larvicidal activity against 3rd instar larvae of important vector mosquitoes i.e. Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti (Diptera: Culicidae). A 24 h bioassay showed that six plant extracts, Acacia nilotica, Argemone mexicana (leaves and seeds), Citrullus colocynthis, Jatropha curcas and Withania somnifera were toxic, with an LC 50 value of less than 100 ppm against all three vector mosquitoes tested.
The vermicomposting ability of Lampito mauritii (Kinberg) and Perionyx ceylanensis Michaelsen was evaluated by using three different types of organic substrates such as leaf litter of Polyalthia longifolia, Pennisetum typhoides cobs (pearl millet) and a weed, Rottboellia exaltata (whole plant except the roots) in combination with cowdung (1:1). Vermicomposting studies (120 days) conducted to optimize the number of worms required for efficient conversion based on the reduction of C/N ratio, percentage decomposition of organic substrates, total number and biomass of earthworms recovered from the vermibed substrates clearly showed that vermibeds with 4 kg of organic materials can hold about 60-80 L. mauritii and about 90-120 P. ceylanensis for efficient decomposition. The percentage decomposition of each organic substrate treated with different numbers of L. mauritii (20, 40, 60, 80 and 100 earthworms) and P. ceylanensis (30, 60, 90, 120 and 150) showed significant difference (P \ 0.001) between numbers of worms introduced per vermibed but the difference between substrates was not significant within the treatments. Vermicomposting resulted in significant increase in electrical conductivity (28.54-49.82%), total nitrogen (43.96-90.83%), total phosphorus (27.42-68.10%) and total potassium (27.42-113.18%), whereas decrease in organic carbon (35.05-49.74%), C/N ratio (55.48-73.18%) and C/P ratio (50.46-66.90%) in different vermibeds introduced with L. mauritii and P. ceylanensis. Both the earthworm species can be used for vermicomposting different organic substrates; however, duration of vermicomposting with P. ceylanensis is not as much of L. mauritii. The use of L. mauritii for vermicomposting of other substrates has been well established by other workers also but standardization of P. ceylanensis, a locally available species, for vermicomposting of different organic substrates is a new finding and the species could be useful for vermiconversion of organic substrates under local conditions.
The study tests whether the lethal effect of overwintering Engelmann spruce seed in its O-horizon affects Douglas-fir, lodgepole pine and subalpine fir seeds and whether the O-horizons of the three associated species have the same lethal effect on seeds. All seed treatments of each species were stratified in petri dishes in the pure O-horizon (unsterilized and autoclaved) of each species. Seed treatments were untreated, 10% slurry of 50% Captan and powdered 75% Captan.All untreated seeds demonstrated the lethal quality of unsterilized Engelmann spruce O-horizon. Its effects on untreated seeds and powder treated seeds were as follows: Engelmann spruce seed 3.5 and 77.3% (germination of untreated and powdered respectively), subalpine fir seed 1.3 and 20.8, Douglas-fir seed 9.5 and 84.8 and lodgepole pine seed 11.9 and 85.7. Subalpine fir O-horizon was lethal to its own seeds but only moderately harmful to seeds of other species. Douglas-fir O-horizon had a significant adverse effect on its own seeds and was only moderately harmful to seeds of other species. Lodgepole pine O-horizon was almost neutral to all seeds. Autoclaving of the O-horizons and powder treatment of seeds produced similar results.
The present study has been conducted to explore the possibility of enrichment of vermicompost with microbial inoculants (i.e., biofertilizer organisms), Azospirillum brasilense and Rhizobium leguminosarum, optimization of inoculum level, and time of inoculation during vermicomposting. The survival rate of each microbial inoculant, total microbial population in vermicompost, and their correlation with the microbial inoculants during the storage period (180 days) were assessed. The change in population of A. brasilense and R. leguminosarum in vermicompost (at 30, 35, and 40 mL/175 g substrates) with reference to storage period showed highly significant negative correlation (P < 0.001). The total microbial population in A. brasilense and R. leguminosarum inoculated vermicompost was high during initial phases of storage and then total microbial population declined towards the end. The inoculum level of A. brasilense and R. leguminosarum at 35 mL per 175 g vermibed substrate is sufficient to maintain 1 × 10 7 viable cells up to 160 days after ther harvesting of vermicompost. The inoculum of these two biofertilizer organisms into vermibed on the 30th day showed increased survival rate and, hence, the optimized inoculation of 35 mL of inoculum per 175 g substrate on the 30th day of vermicomposting is helpful for the maintenance of sufficient viable population for more than five months in the enriched vermicompost.
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