Human-driven changes in nitrogen (N) and phosphorus (P) inputs are modifying biogeochemical cycles and the trophic state of many habitats worldwide. These alterations are predicted to continue to increase, with the potential for a wide range of impacts on invertebrates, key players in ecosystem-level processes. Here, we present a meta-analysis of 1679 cases from 207 studies reporting the effects of N, P, and combined N + P enrichment on the abundance, biomass, and richness of aquatic and terrestrial invertebrates. Nitrogen and phosphorus additions decreased invertebrate abundance in terrestrial and aquatic ecosystems, with stronger impacts under combined N + P additions. Likewise, N and N + P additions had stronger negative impacts on the abundance of tropical than temperate invertebrates. Overall, the effects of nutrient enrichment did not differ significantly among major invertebrate taxonomic groups, suggesting that changes in biogeochemical cycles are a pervasive threat to invertebrate populations across ecosystems. The effects of N and P additions differed significantly among invertebrate trophic groups but N + P addition had a consistent negative effect on invertebrates. Nutrient additions had weaker or inconclusive impacts on invertebrate biomass and richness, possibly due to the low number of case studies for these community responses. Our findings suggest that N and P enrichment affect invertebrate community structure mainly by decreasing invertebrate abundance, and these effects are dependent on the habitat and trophic identity of the invertebrates. These results highlight the important effects of human-driven nutrient enrichment on ecological systems and suggest a potential driver for the global invertebrate decline documented in recent years.

Recent studies have documented global declines in insects and their relatives, but the exact mechanisms explaining these patterns are not fully understood.A potential driver underlying arthropod population declines is increases in anthropogenic inputs of nitrogen (N) and phosphorus (P). Here, we synthesize the effects of N, P, and combined N + P enrichment on the abundance of hexapods (insects and collembola) and arachnids from 901 experiments reported in 84 studies. We found that N and combined N + P enrichment caused significant decreases in the abundance of these groups overall. While arthropod responses to nutrient enrichment across aquatic and terrestrial habitats and in temperate as well as tropical climatic zones differed in magnitude, our results suggest that arthropods are decreasing similarly in response to nitrogen and phosphorus enrichment. Further, despite previously shown differences in the nutrient demands of different insect metamorphosis groups, we found consistent negative effects of N + P enrichment on all groups. Our results also showed that the negative effects of nutrient additions are stronger for aquatic insects that are considered more sensitive to changes in physical-chemical parameters in their environments, Ephemeroptera, Plecoptera, and Trichoptera (EPT), compared with other aquatic insects. In addition, N + P enrichment reduced the abundance of above-ground and below-ground arthropods, suggesting that a similar mechanism driving arthropod community change is acting on both groups. These findings suggest that changes in elemental cycles are a potential cause of the ongoing global decline of arthropods and underscore the serious effects of nutrient enrichment on ecological systems.