The investigation provides experimental evidence that the unbiodegradable particulate organics fractions of primary sludge and waste activated sludge calculated from activated sludge models remain essentially unbiodegradable in anaerobic digestion. This was tested by feeding the waste activated sludge (WAS) from three different laboratory activated sludge (AS) systems to three separate anaerobic digesters (AD). Two of the AS systems were Modified Ludzack - Ettinger (MLE) nitrification-denitrification (ND) systems and the third was a membrane University of Cape Town (UCT) ND and enhanced biological P removal system. One of the MLE systems and the UCT system were fed the same real settled wastewater. The other MLE system was fed raw wastewater which was made by adding a measured constant flux (gCOD/d) of macerated primary sludge (PS) to the real settled wastewater. This PS was also fed to a fourth AD and a blend of PS and WAS from settled wastewater MLE system was fed to a fifth AD. The five ADs were each operated at five different sludge ages (10-60d). From the measured performance results of the AS systems, the unbiodegradable particulate organic (UPO) COD fractions of the raw and settled wastewaters, the PS and the WAS from the three AS systems were calculated with AS models. These AS model based UPO fractions of the PS and WAS were compared with the UPO fractions calculated from the performance results of the ADs fed these sludges. For the PS, the UPO fraction calculated from the AS and AD models matched closely, i.e. 0.30 and 0.31. Provided the UPO of heterotrophic (OHO, fE_OHO) and phosphorus accumulating (PAO, fE_PAO) biomass were accepted to be those associated with the death regeneration model of organism "decay", the UPO of the WAS calculated from the AS and AD models also matched well - if the steady state AS model fE_OHO = 0.20 and fE_PAO = 0.25 values were used, then the UPO fraction of the WAS calculated from the AS models deviated significantly from those calculated with the AD models. Therefore in plant wide wastewater treatment models the characterization of PS and WAS as defined by the AS models can be applied without modification in AD models. The observed rate limiting hydrolysis/acidogenesis rates of the sludges are listed.

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Biological wastewater treatment processes such as activated sludge and anaerobic digestion remain the most favorable when compared to processes such as chemical precipitation and ion exchange due to their cost-effectiveness, eco-friendliness, ease of operation, and low maintenance. Since Abattoir Wastewater (AWW) is characterized as having high organic content, anaerobic digestion is slow and inadequate for complete removal of all nutrients and organic matter when required to produce a high-quality effluent that satisfies discharge standards. Multi-integrated systems can be designed in which additional stages are added before the anaerobic digester (pre-treatment), as well as after the digester (post-treatment) for nutrient recovery and pathogen removal. This can aid the water treatment plant effluent to meet the discharge regulations imposed by the legislator and allow the possibility for reuse on-site. This review aims to provide information on the principles of anaerobic digestion, aeration pre-treatment technology using enzymes and a hybrid membrane bioreactor, describing their various roles in AWW treatment. Simultaneous nitrification and denitrification are essential to add after anaerobic digestion for nutrient recovery utilizing a single step process. Nutrient recovery has become more favorable than nutrient removal in wastewater treatment because it consumes less energy, making the process cost-effective. In addition, recovered nutrients can be used to make nutrient-based fertilizers, reducing the effects of eutrophication and land degradation. The downflow expanded granular bed reactor is also compared to other high-rate anaerobic reactors, such as the up-flow anaerobic sludge blanket (UASB) and the expanded granular sludge bed reactor (EGSB).

The feasibility of a biological (EcoflushTM) and/or electrocoagulation (EC) treatment system in removing chemical oxygen demand (COD) and fats, oils, and grease (FOG) from poultry slaughterhouse wastewater (PSW) were studied. The response surface methodology (RSM) was used to identify the optimum operating condition for EC and its integration with EcoflushTM as a pre-treatment for the removal of lipids. The optimum operating conditions were obtained at a pH of 3.05, a current density of 66.9 A/m2, 74-min of treatment time, and without Ecoflush™. These conditions produced a high-quality clarified effluent after 92.4% COD reduction and 99% FOG reduction. The treatment with EcoflushTM only resulted in 85–99% FOG reduction, 20–50% COD reduction, and odourless effluent. However, the combination of both processes (EcoflushTM and EC) did not yield a significant difference (F test, p > 0.05) when compared to the performance of EC alone. Despite the low removal percentages of nitrogen and phosphorus, the present study proved that EC is an effective method for the removal of COD and FOG, rendering an effluent that meets the permissible discharge standards for the City of Cape Town. The novel Ecoflush™ also proved to be very efficient in the removal of FOG from PSW.

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