Zooplankton plays a major role in ocean food webs and biogeochemical cycles, and provides major ecosystem services as a main driver of the biological carbon pump and in sustaining fish communities. Zooplankton is also sensitive to its environment and reacts to its changes. To better understand the importance of zooplankton, and to inform prognostic models that try to represent them, spatially-resolved biomass estimates of key plankton taxa are desirable. In this study we predict, for the first time, the global biomass distribution of 19 zooplankton taxa (1-50 mm Equivalent Spherical Diameter) using observations with the Underwater Vision Profiler 5, a quantitative in situ imaging instrument. After classification of 466,872 organisms from more than 3,549 profiles (0-500 m) obtained between 2008 and 2019 throughout the globe, we estimated their individual biovolumes and converted them to biomass using taxa-specific conversion factors. We then associated these biomass estimates with climatologies of environmental variables (temperature, salinity, oxygen, etc.), to build habitat models using boosted regression trees. The results reveal maximal zooplankton biomass values around 60°N and 55°S as well as minimal values around the oceanic gyres. An increased zooplankton biomass is also predicted for the equator. Global integrated biomass (0-500 m) was estimated at 0.403 PgC. It was largely dominated by Copepoda (35.7%, mostly in polar regions), followed by Eumalacostraca (26.6%) Rhizaria (16.4%, mostly in the intertropical convergence zone). The machine learning approach used here is sensitive to the size of the training set and generates reliable predictions for abundant groups such as Copepoda (R2 ≈ 20-66%) but not for rare ones (Ctenophora, Cnidaria, R2 < 5%). Still, this study offers a first protocol to estimate global, spatially resolved zooplankton biomass and community composition from in situ imaging observations of individual organisms. The underlying dataset covers a period of 10 years while approaches that rely on net samples utilized datasets gathered since the 1960s. Increased use of digital imaging approaches should enable us to obtain zooplankton biomass distribution estimates at basin to global scales in shorter time frames in the future.

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As the basis of oceanic food webs and a key component of the biological carbon pump, planktonic organisms play major roles in the oceans. Their study benefited from the development of in situ imaging instruments, which provide higher spatio-temporal resolution than previous tools. But these instruments collect huge quantities of images, the vast majority of which are of marine snow particles or imaging artifacts. Among them, the In Situ Ichthyoplankton Imaging System (ISIIS) samples the largest water volumes (> 100 L s-1) and thus produces particularly large datasets. To extract manageable amounts of ecological information from in situ images, we propose to focus on planktonic organisms early in the data processing pipeline: at the segmentation stage. We compared three segmentation methods, particularly for smaller targets, in which plankton represents less than 1% of the objects: (i) a traditional thresholding over the background, (ii) an object detector based on maximally stable extremal regions (MSER), and (iii) a content-aware object detector, based on a Convolutional Neural Network (CNN). These methods were assessed on a subset of ISIIS data collected in the Mediterranean Sea, from which a ground truth dataset of > 3,000 manually delineated organisms is extracted. The naive thresholding method captured 97.3% of those but produced ~340,000 segments, 99.1% of which were therefore not plankton (i.e. recall = 97.3%, precision = 0.9%). Combining thresholding with a CNN missed a few more planktonic organisms (recall = 91.8%) but the number of segments decreased 18-fold (precision increased to 16.3%). The MSER detector produced four times fewer segments than thresholding (precision = 3.5%), missed more organisms (recall = 85.4%), but was considerably faster. Because naive thresholding produces ~525,000 objects from 1 minute of ISIIS deployment, the more advanced segmentation methods significantly improve ISIIS data handling and ease the subsequent taxonomic classification of segmented objects. The cost in terms of recall is limited, particularly for the CNN object detector. These approaches are now standard in computer vision and could be applicable to other plankton imaging devices, the majority of which pose a data management problem.

AimThe distribution of mesoplankton communities has been poorly studied at global scale, especially from in situ instruments. This study aims to (1) describe the global distribution of mesoplankton communities in relation to their environment and (2) assess the ability of various environmental‐based ocean regionalizations to explain the distribution of these communities.LocationGlobal ocean, 0–500 m depth.Time Period2008–2019.Major Taxa StudiedTwenty‐eight groups of large mesoplanktonic and macroplanktonic organisms, covering Metazoa, Rhizaria and Cyanobacteria.MethodsFrom a global data set of 2500 vertical profiles making use of the Underwater Vision Profiler 5 (UVP5), an in situ imaging instrument, we studied the global distribution of large (>600 μm) mesoplanktonic organisms. Among the 6.8 million imaged objects, 330,000 were large zooplanktonic organisms and phytoplankton colonies, the rest consisting of marine snow particles. Multivariate ordination (PCA) and clustering were used to describe patterns in community composition, while comparison with existing regionalizations was performed with regression methods (RDA).ResultsWithin the observed size range, epipelagic plankton communities were Trichodesmium‐enriched in the intertropical Atlantic, Copepoda‐enriched at high latitudes and in upwelling areas, and Rhizaria‐enriched in oligotrophic areas. In the mesopelagic layer, Copepoda‐enriched communities were also found at high latitudes and in the Atlantic Ocean, while Rhizaria‐enriched communities prevailed in the Peruvian upwelling system and a few mixed communities were found elsewhere. The comparison between the distribution of these communities and a set of existing regionalizations of the ocean suggested that the structure of plankton communities described above is mostly driven by basin‐level environmental conditions.Main ConclusionsIn both layers, three types of plankton communities emerged and seemed to be mostly driven by regional environmental conditions. This work sheds light on the role not only of metazoans, but also of unexpected large protists and cyanobacteria in structuring large mesoplankton communities.

Doliolids are common gelatinous grazers in marine ecosystems around the world and likely influence carbon cycling due to their large population sizes with high growth and excretion rates. Aggregations or blooms of these organisms occur frequently, but they are difficult to measure or predict because doliolids are fragile, under sampled with conventional plankton nets, and can aggregate on fine spatial scales (1-10 m). Moreover, ecological studies typically target a single region or site that does not encompass the range of possible habitats favoring doliolid proliferation. To address these limitations, we combined in situ imaging data from six coastal ecosystems, including the Oregon shelf, northern California, southern California Bight, northern Gulf of Mexico, Straits of Florida, and Mediterranean Sea, to resolve and compare doliolid habitat associations during warm months when environmental gradients are strong and doliolid blooms are frequently documented. Higher ocean temperature was the strongest predictor of elevated doliolid abundances across ecosystems, with additional variance explained by chlorophyll a fluorescence and dissolved oxygen. For marginal seas with a wide range of productivity regimes, the nurse stage tended to comprise a higher proportion of the doliolids when total abundance was low. However, this pattern did not hold in ecosystems with persistent coastal upwelling. The doliolids tended to be most aggregated in oligotrophic systems (Mediterranea and southern California), suggesting that microhabitats within the water column favor proliferation on fine spatial scales. Similar comparative approaches can resolve the realized niche of fast-reproducing marine animals, thus improving predictions for population-level responses to changing oceanographic conditions.The environmental conditions associated with animal aggregations or population growth can indicate suitable or favorable habitat (Pulliam 2000; Treible et al. 2022).Describing these conditions is a necessary first step toward predicting how populations will respond to environmental change. For most marine animals, however, environmental