Limited information is available regarding the adhesion to eroded dentin. This study aims to evaluate the effect of different surface treatments on eroded dentin morphology and on microtensile bond strength (μTBS) of adhesive systems to this substrate. Ninety-six extracted third molars were randomly divided into eight groups (n = 12) according to the type of surface treatment and the adhesive system: G1 = Control + Clearfil SE Bond [SE], G2 = Diamond bur [DB] + SE, G3 = Er:YAG laser (60 mJ, 2 Hz, 0.12 W, 19.3 J/ cm(2)) + SE, G4 = Er,Cr:YSGG laser (50 mJ, 30 Hz, 1.5 W, 4.5 J/ cm(2)) + SE, G5 = Control + Single Bond [SB], G6 = DB + SB, G7 = Er:YAG + SB, G8 = Er,Cr:YSGG + SB. The erosive cycling was performed by immersion in 0.05 M citric acid (pH 2.3, 10 min, 6x/day) and in supersaturated solution (pH 7.0, 1 h, between acid attacks), during 5 days. Blocks of composite were bonded to the samples according to the manufacturers' instructions. After 24 h-storage in distilled/deionized water (37 °C), stick-shaped samples were obtained and submitted to μTBS test. Each surface treatment was analyzed under scanning electron microscopy (n = 4) and the bond strength values (megapascal) were analyzed by two-way ANOVA and Tukey tests (α = 0.05). All surface treatments lead to changes on eroded dentin. G4 showed the highest bond strength mean (28.3 ± 9.2 MPa), which was statistically significant higher than all the other groups (p < 0.05). The surface treatment with Er,Cr:YSGG laser irradiation (4.5 J/cm(2)/50 mJ/30 Hz/140 μs) prior to bonding with a self-etching adhesive system significantly increases adhesion to eroded dentin, as compared to conventional treatment.
The aim of this in vitro study was to evaluate the effect of different surface treatments (control, diamond bur, erbium-doped yttrium aluminum garnet (Er:YAG) laser, and erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser) on sound dentin surface morphology and on microtensile bond strength (μTBS). Sixteen dentin fragments were randomly divided into four groups (n = 4), and different surface treatments were analyzed by scanning electron microscopy. Ninety-six third molars were randomly divided into eight groups (n = 12) according to type of surface treatment and adhesive system: G1 = Control + Clearfil SE Bond (SE); G2 = Control + Single Bond (SB); G3 = diamond bur (DB) + SE; G4 = DB + SB, G5 = Er:YAG laser (2.94 μm, 60 mJ, 2 Hz, 0.12 W, 19.3 J/cm(2)) + SE; G6 = Er:YAG + SB, G7 = Er,Cr:YSGG laser (2.78 μm, 50 mJ, 30 Hz, 1.5 W, 4.5 J/cm(2)) + SE; and G8 = Er,Cr:YSGG + SB. Composite blocks were bonded to the samples, and after 24-h storage in distilled/deionized water (37 °C), stick-shaped samples were obtained and submitted to μTBS test. Bond strength values (in megapascal) were analyzed by two-way ANOVA and Tukey tests (α = 0.05). G1 (54.69 ± 7.8 MPa) showed the highest mean, which was statistically significantly higher than all the other groups (p < 0.05). For all treatments, SE showed higher bond strength than SB, except only for Er,Cr:YSGG treatment, in which the systems did not differ statistically from each other. Based on the irradiation parameters considered in this study, it can be concluded that Er:YAG and Er,Cr:YSGG irradiation presented lower values than the control group; however, their association with self-etching adhesive does not have a significantly negative effect on sound dentin (μTBS values of >20 MPa).
Although several studies have demonstrated the efficacy of AmF/NaF/SnCl2 solution in inhibiting dental erosion progression, measures for further improvement in its effectiveness are paramount. Thus, this in situ study evaluated whether the protective effect promoted by the AmF/NaF/SnCl2 solution would be enhanced by increasing its frequency of use. The study was conducted with 12 volunteers, a 4-phase (5 days each) randomized, crossover model. Extraoral erosive challenges (0.5% citric acid, pH 2.6, 6 × 2 min/day) and rinsing protocol (1 or 2 × 2 min/day) were performed. Before the in situ phase, human enamel samples were subjected to an in vitro surface softening (1% citric acid, pH 4.0, for 3 min). Four treatment protocols were tested using samples in replicas (n = 12): group G1 - deionized water (negative control); G2 - NaF solution (positive control, 500 ppm F-, pH 4.5); G3 - AmF/NaF/SnCl2 solution (500 ppm F-, 800 ppm Sn2+, pH 4.5) once a day; G4 - AmF/NaF/SnCl2 solution twice a day. Tissue loss and morphological changes were determined by optical profilometry (n = 12) and scanning electron microscopy (n = 3) analysis, respectively. Data were statistically analyzed by ANOVA with subsequent pairwise comparison of treatments. Tissue loss means (±SD in µm) for each treatment protocol and statistical differences were found as follows: G1 4.55 ± 2.75, G2 4.59 ± 2.13, G3 2.64 ± 1.55, and G4 1.34 ± 1.16. Although there was no difference between the 2 AmF/NaF/SnCl2 solution application regimens (once or twice a day), application of the product twice a day was the only treatment that was able to control erosion progression, differing from the control groups.
This in vitro study aimed to investigate the potential of CO 2 lasers associated with different fluoride agents in inhibiting enamel erosion. Human enamel samples were randomly divided into 9 groups (n = 12): G1-eroded enamel; G2-APF gel; G3-AmF/NaF gel; G4-AmF/SnF 2 solution; G5-CO 2 laser (λ = 10.6 µm)+APF gel; G6-CO 2 laser+AmF/NaF gel; G7-CO 2 laser+AmF/SnF 2 solution; G8-CO 2 laser; and G9-sound enamel. The CO 2 laser parameters were: 0.45 J/cm 2 ; 6 μs; and 128 Hz. After surface treatment, the samples (except from G9) were immersed in 1% citric acid (pH 4.0, 3 min). Surface microhardness was measured at baseline and after surface softening. The data were statistically analyzed by oneway ANOVA and Tukey's tests (p < 0.05). G2 (407.6 ± 37.3) presented the highest mean SMH after softening, followed by G3 (407.5 ± 29.8) and G5 (399.7 ± 32.9). Within the fluoride-treated groups, G4 (309.0 ± 24.4) had a significantly lower mean SMH than G3 and G2, which were statistically similar to each other. AmF/NaF and APF application showed potential to protect and control erosion progression in dental enamel, and CO 2 laser irradiation at 0.45J/cm 2 did not influence its efficacy. CO 2 laser irradiation alone under the same conditions could also significantly decrease enamel erosive mineral loss, although at lower levels.
This in situ study aimed to investigate the effect of a tin-containing fluoride solution in preventing enamel erosion. Also, its effects on the partly demineralized zone were assessed for the first time. Thirteen volunteers participated in this 2-phase study, wearing removable intra-oral appliances containing four sterilized bovine enamel slabs, for 8 days, where 2 treatment protocols were tested using samples in replicas (n = 13): CO -no treatment (negative control) and FL -AmF/NaF/SnCl 2 solution (500 ppm F-, 800 ppm Sn 2+ , pH = 4.5). Samples were daily exposed to an erosive challenge (0.65% citric acid, pH 3.6, 4 min, 2x/day). In the 2 nd phase, volunteers switched to the other treatment protocol. Samples were evaluated for surface loss using a profilometer (n = 13) and a cross-sectional nanohardness (CSNH) test (n = 13) was carried out in order to determine how deep the partly demineralized zone reaches below the erosive lesion. The data were statistically analyzed by two-way ANOVA. Erosive challenges lead to smaller enamel surface loss (p < 0.001) in the FL group when compared to group CO. Data from CSNH showed that there was no significant difference in demineralized enamel zone underneath erosion lesions between the groups. An amorphous layer could be observed on the surface of enamel treated with tin-containing solution alone. Under the experimental conditions of this in situ study, it can be concluded that AmF/NaF/SnCl 2 solution prevents enamel surface loss but does not change the hardness of the partly demineralized zone near-surface enamel.
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