The germination response of seeds under artificial stress conditions is a tool for better understanding of the survival and adaptation ability of species under natural stress conditions. The aims of this study were to evaluate the protective effect of nitric oxide during germination as well as seed vigor and seedling development of Eucalyptus urophylla under salt stress conditions. The experimental design was completely randomized, with five replications, in a 3 × 3 + 1 factorial arrangement, with three priming factors [water, sodium nitroprusside (SNP), and potassium nitrate (KNO3)], three germination conditions (distilled water, and the water potentials of -0.6 and -1.2 MPa), and an additional control treatment (unprimed seeds). The following determinations were made: germination count at seven and fourteen days, germination speed index, primary root length, shoot length, and total length. SNP protects seeds, leading to a higher percentage of germination, as well as greater root growth and total seedling size, whereas KNO3 is not effective in protecting seeds that suffer from salt stress, which affects their physiological and morphological characteristics. E. urophylla seeds are sensitive to salt stress, and physiological priming with SNP leads to an increase in the percentage of germination, vigor, and seedling development under salinity conditions.
Several studies have successfully used polyethylene glycol (PEG) and/or abscisic acid (ABA) to reinduce desiccation tolerance in germinated seeds of orthodox species, improving our understanding of the mechanisms that underlie this trait. In this context, treatments with PEG and ABA were tested to assess their efficiency in reducing the sensitivity to desiccation and increasing the storage period of Magnolia ovata seeds. The drying of seeds to 10% water did not significantly affect germination, nor was germination impaired in seeds that were dried and treated with ABA. However, seeds treated with PEG had lower viability. After storage, ABA-treated seeds with as low as 5% water content (WC) had higher germination rates, the treatment with 100 µM ABA providing the greatest storage capacity. No changes were observed in the activity of catalase, peroxidase, or superoxide dismutase or in the abundance of total or heat-resistance proteins.
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