ResumoO plasma rico em plaquetas (PRP) possui diversos fatores de crescimento que auxiliam na regeneração tecidual. O presente trabalho tem como objetivo propor uma técnica para o preparo do PRP autólogo, por meio de um protocolo simples e rápido, obtido a partir de um volume pequeno de sangue total, que facilita a utilização em cães. Após a colheita de 8ml de sangue total, realizada em cinco cães, obtevese o PRP, de maneira asséptica, com uma concentração de plaquetas de duas a seis vezes maior que a contagem sérica inicial, com valores absolutos finais de 800.000 a 1.600.000/mm³. A técnica pode ser realizada sem complicações, de maneira rápida, com poucos equipamentos, atingindo-se a concentração ideal das plaquetas e mantendo-se a esterilidade do plasma.
Palavras-chave: Plasma rico em plaquetas, regeneração tecidual, cão
AbstractPlatelet-rich plasma (PRP) has many growing factors that improve tissue regeneration. The goal of this paper is to describe an innovative, quick and simple technique for preparing autogenous PRP from a small amount of blood, which allowing use in dogs. After harvesting 8ml of total blood in five dogs, PRP was obtained using an aseptic technique, which rendered a platelet count two to six times more concentrated than the previous serum platelet count, with final absolute values between 800.000 and 1.600.000/mm 3 . The technique can be developed quickly, without complications using simple equipments, reaching an ideal platelet concentration and keeping plasma sterility.
Porcine group C rotavirus (RVC) is recognised as an enteric pathogen in piglets worldwide. The VP6 gene of RVC is divided into seven I-genotypes. Genotypes I2 and I3 are found in human and bovine strains, respectively; the porcine strains are divided into the other five genotypes (I1, I4-I7). In this study, molecular analysis of nearly the full length of the VP6 gene was performed in 11 Brazilian wild-type porcine RVC strains identified in diarrhoeic faecal samples, which were collected from eight pig farms located in five Brazilian states from piglets of 1-4 weeks of age. The nucleotide sequences of the VP6 gene showed 82.9-100 % identity between the Brazilian strains, 84.9-93.1 % with the prototype Cowden strain, and 82.4-92.2 % with other porcine RVC strains. In the 11 diarrhoeic faecal samples analysed in this study, three distinct porcine RVC genotypes (I1, I5, and I6) were identified and none were predominant. The results presented in this study revealed a high nucleotide diversity of the VP6 gene in porcine RVC field strains circulating in Brazil, which highlights the importance of further epidemiological and molecular surveys worldwide.
The aim of this study was to perform the molecular characterization of the eleven genes of a G6P[5] bovine group A rotavirus (RVA) strain detected in a diarrhea outbreak from a vaccinated beef cattle herd. The outbreak affected 80 % of calves between 15-30 days old. RVA was identified by RT-PCR in 12 (70.6 %) out of 17 diarrheic fecal samples evaluated. The rotavirus wild-type strain had the genotype constellation G6(IV)-P[5](IX)-I2c-R2-C2-M2-A3-N2-T6-E2e-H3a. This study confirms the importance of homotypic immunity against the bovine RVA P[5] genotype in neonatal diarrhea in cattle herds that are regularly vaccinated against rotaviruses.
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