G and P genotype profiles of rotavirus A field strains circulating in beef and dairy cattle herds in Brazil, 2006–2015

Medeiros, Thais Neris da Silva; Lorenzetti, Elis; Alfieri, Amauri Alcindo

doi:10.1016/j.cimid.2019.03.002

Cited by 15 publications

(51 citation statements)

References 38 publications

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“…Rotavirus A was detected in feces and intestinal contents collected from dairy and beef calves with a frequency of 57%, which was higher than reports from Argentina and Brazil (17–42%) [ 19 , 20 , 21 , 22 ], and other geographic regions (20–49%) [ 7 , 23 , 24 , 25 ]. On the other hand, in Australia, the frequency of RVA detection was 80%, which is higher than the detected in our study [ 6 ].…”

Section: Discussionmentioning

confidence: 56%

Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission

et al. 2020

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Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.

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Section: Discussionmentioning

confidence: 56%

Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission

et al. 2020

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“…The infectious disease pathogens associated with the development of NCD can be divided into viral, bacterial, and protozoa agents. Rotavirus A (RVA), bovine coronavirus (BCoV), bovine kobuvirus (BKV), and bovine viral diarrhea virus 1 and 2 (BVDV-1 and BVDV-2) are the more frequently viral pathogens associated with the development of NCD [6][7][8][9][10]. The most common bacterial agents are enteropathogenic Escherichia coli (ETEC) and Salmonella sp.…”

Section: Introductionmentioning

confidence: 99%

“…Aliquots of sterile ultrapure diethylpyrocarbonate-treated water were included as negative controls in all procedures. Samples previously known as positive for each of the virus investigated in this study were included as positive controls: prototype NADL and Mebus strains cell culture (Madin-Darby bovine kidney) adapted for BVDV and BCoV, respectively, and fecal samples known to be positive for RVA [9] and BKV [21].…”

Section: Introductionmentioning

confidence: 99%

Severe outbreak of bovine neonatal diarrhea in a dairy calf rearing unit with multifactorial etiology

et al. 2021

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This study describes the etiological diversity observed in a severe neonatal diarrhea outbreak with morbidity and mortality rates of 80 and 20%, respectively, with detection of mixed infections with viral, bacterial, and protozoan disease agents in a dairy calf rearing unit. Diarrheic fecal samples were collected from eight 5 to 18 days of age calves and were submitted to the investigation of the presence of rotavirus A (RVA), bovine coronavirus (BCoV), bovine kobuvirus (BKV), bovine viral diarrhea virus 1 and 2 (BVDV-1 and BVDV-2), enteropathogenic Escherichia coli (ETEC), Salmonella sp., and Cryptosporidium spp. Fragments of the small intestine of one calf with diarrhea that spontaneously died were submitted for histopathological analyses. The most frequent infectious agent detected in diarrheic fecal samples was BKV (8/8-100%), followed by RVA (5/8-62.5%), BVDV (5/8-62.5%), Cryptosporidium parvum (5/8-62.5%), ETEC (4/8-50%), and Cryptosporidium ryanae (1/8-12.5%). These etiological agents were found in mixed infections with two or more pathogens per diarrheic fecal sample. The association of viral and protozoan pathogens was the most frequently identified (37.5%) in these samples, followed by viral and bacterial (25%); viral, bacterial, and protozoan (25%); and only viral agents (12.5%). BCoV and Salmonella sp. were not identified in the diarrheic fecal samples analyzed. Additionally, histopathology of the small intestine diagnosed chronic lymphocytic enteritis. In conclusion, in calf rearing units, the adoption and strict monitoring of health management practices are critical to the success of this calf creation system.

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“…1 RVs are divided into nine serogroups named from A to I, based on the nucleotide sequence, antigenic characteristics of the VP6 protein, and the migration pattern of their genomic fragments in gel electrophoresis. 2 Rotavirus A (RVA) is the most important serogroup and it is the main cause of diarrhea in newborns of humans and calves. RVs group B (RVB) and C (RVC) are also important in humans and some animals.…”

Section: Introductionmentioning

confidence: 99%

“…Common human genotypes include G1-4 and G9 in combination with P [4], P [6], and P [8]. 2 Since some reports indicate that RVA can act as a potential zoonosis, numerous cases of pathogenicity of RVA in cattle and other animals have been reported that were isolated from diarrheic children [12][13][14] ; As a complementary part of this study, fecal specimens of calves were examined using specific primers of common human G and P genotypes to give a better insight into the epidemiology of RVA in Iran.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran

Nazaktabar

Madadgar

2020

Int J Enteric Pathog

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Background: Rotavirus (RV) is one of the most important causes of diarrhea in the calf and human neonates. Rotaviruses are divided into nine different serogroups, of which group A is more important compared to other groups. Objective: This study was performed because of the lack of information about the importance and prevalence of bovine rotaviruses B (RVB) and C (RVC) and human genotypes of rotavirus A (RVA) in the bovine population in Iran. Phylogenetic analysis of VP6 of bovine RVA was the second aim of the present study. Materials and Methods: A total of 581 stool specimens were collected from diarrheic calves of 14 provinces and were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and 485 of them were investigated by PAGE electrophoresis to determine the frequency of three rotaviruses A (RVA), B (RVB), and C (RVC). The presence of human G and P genotypes in Iranian bovine population was also evaluated using semi-nested multiplex RT-PCR. Results: RVA was detected by RT-PCR (VP6 gene detection) in 16.2% (94/581) and by PAGE in 22.16% (108/485) and no positive cases of RVB and RVC were confirmed by polyacrylamide gel electrophoresis (PAGE). This study showed that non-A RV groups (B and C) have little role in calf diarrhea in Iran. The results of the phylogenetic study of VP6 sequences of rotaviruses A identified in this study showed that they all belonged to genotype I2 and were classified into three different branches. Specimen isolated in Zanjan showed the highest difference (maximum identity of 94%) with other sequences and clustered along with the Japanese strain, R22. Human G and P genotypes were not found in the studied samples. Conclusion: The results showed that non-A rotaviruses and human genotypes of RVA are of little importance in calf rotavirus diarrhea in Iran. Also, there is the first phylogenetic study of rotavirus A VP6 protein in Iran.

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G and P genotype profiles of rotavirus A field strains circulating in beef and dairy cattle herds in Brazil, 2006–2015

Cited by 15 publications

References 38 publications

Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission

Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission

Severe outbreak of bovine neonatal diarrhea in a dairy calf rearing unit with multifactorial etiology

Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran

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