Background The impact of the COVID‐19 pandemic on behavioural and psychosocial aspects related to oral health is unknown. Aim This study evaluated the psychosocial and behavioural changes related to oral health in adolescents immediately before and during the pandemic period of COVID‐19, enabling a longitudinal assessment of the perceived changes. Design This cohort study evaluated 290 adolescents from November 2019 to February 2020 (T1—before the pandemic in Brazil) and from June to July 2020 (T2) in southern Brazil. Sociodemographic, behavioural, and psychosocial variables were measured before and during the pandemic. Issues related to social distancing and job loss were also collected. The differences between the variables in T1 and T2, as well as the effect of social distancing, were assessed using a multilevel‐adjusted logistic regression model for repeated measures. Results A total of 207 adolescents were re‐evaluated at T2 (a response rate of 71.3%). During the pandemic, the frequency of toothbrushing, the use of dental services, and the self‐perceived need for dental treatment significantly decreased. Sugar consumption, bruxism, and quality of sleep did not change significantly. Conclusion Behavioural and psychosocial factors showed significant changes due to the COVID‐19 pandemic in adolescents.

Skin color played an important role in dentists' treatment decisions. Professionals may contribute unconsciously to the propagation and replication of racial discrimination.

Resumo Introdução A saúde pública no Brasil sofreu grandes mudanças nas últimas décadas. Objetivo Descrever o panorama da produção odontológica realizada pelo SUS de 1999 a 2017 no Brasil e suas macrorregiões. Método Os dados foram obtidos no Sistema de Informação Ambulatorial (SIA-SUS) e do Instituto Brasileiro de Geografia e Estatística (IBGE). Foram criadas taxas de procedimentos (por 100 mil habitantes/ano) realizados em cada macrorregião: procedimentos restauradores, protéticos, coletivos, endodontia, exodontia, periodontia e preventivos de 1999 a 2017. A análise estatística das séries temporais foi realizada utilizando um modelo de regressão linear. Resultados Procedimentos protéticos e de periodontia foram os únicos que apresentaram uma tendência linear positiva em todas as macrorregiões brasileiras (p<0,001). A Endodontia não apresentou tendência positiva no Brasil (p=0,173). Restaurações apresentaram um crescimento na macrorregião Norte (p=0,003) e Centro-Oeste (p<0,001). Exodontias apresentaram na macrorregião Norte uma tendência de aumento (p=0,046) enquanto que, no Centro-Oeste, apresentaram uma diminuição (p=0,049). Procedimentos preventivos (p=0,042) e coletivos (p=0,017) apresentaram uma diminuição da sua produção durante o período. Conclusão A saúde bucal apresentou um grande crescimento dentro do Sistema Único de Saúde nos 19 anos avaliados. Procedimentos de periodontia e de prótese dentária foram aqueles com as maiores tendências de crescimento.

The aim of this study was to evaluate the proliferation and adhesion of mesenchymal cells (3T3/NIH) in Dulbecco’s Modified Eagle Medium(DMEM) supplemented with Platelet-Poor Plasma (PPP) in aPlatelet-Rich Fibrin (PRF) scaffold. Human blood was obtained and processed in a centrifuge considering the equation G=1.12xRx(RPM/1000)2 to obtain PRF and PPP.Cell adhesion and maintenance analyses were performed by MTTassays in a 96 well plate withsupplemented DMEM: PPP (90:10) for 24 hours. Besides, the PRF was deposited in a 48 well plate and 10x104 cells were seeded above each PRF (n=3) with 800µl of DMEM: PPP (90:10) and cultured for 7 days. Histological analysis and the immunohistochemical staining for Vimentin were performed. Results were analyzed by one-way ANOVA in Stata12®. A significant decrease (p<0.05) of cells adhesion in relationship to FBSwas observed. However, a similar ability of cell-maintenance for PPP 10% was observed (P>0.05). Fibroblasts culture for 7 days in PRF supplemented with PPP 10% was possible, showing positive staining for Vimentin. Therefore, PPP cell supplementation decreased the initial adhesion of cells but was able to maintain the proliferation of adhered cells and able to support their viability in PRF.It seems that this method has many clinical advantagessince it provides an autologous and natural scaffold with their respective supplement for cell culture by only one process, without using xenogeneic compounds. This could improve the potential of clinical translational therapies based on the use of PRF cultured cells, promoting the regenerative potential for future use in medicine and dentistry.

Background/Aim Knowledge of factors influencing the occurrence of crown discoloration following traumatic dental injuries in primary teeth and understanding its possible consequences can help dentists to provide appropriate care. The aim of this retrospective cohort study was to investigate the incidence of crown discoloration following trauma in primary teeth. This study also estimated the risk of pulp necrosis with subsequent infection of the root canal system and pulp canal obliteration in discolored teeth. Material and Methods Records of children treated at the specialized trauma center of the Federal University of Pelotas during a period of 10 years were analyzed. Patient details including the type of injury, presence of crown discoloration, pulp necrosis, and pulp canal obliteration were collected. Photographs of teeth with crown discoloration were assessed to evaluate the color presented (yellow or dark). Chi‐square tests and Poisson regression were used for data analyses (P < .05). Results A total of 355 children with 628 traumatized teeth were included in the study. The incidence of crown discoloration was 27.9%, and 20.5% of the teeth were affected. Discoloration was higher in children whose age at the time of trauma was 2‐4 years (P = .020), and it was associated with the type of injury (P = .019). Discolored teeth had a twofold risk of pulp necrosis and subsequent infection of the root canal system (RR 1.98 95% CI 1.42‐276; P < .001) and a higher risk of pulp canal obliteration (RR 8.47; 95% CI 4.95‐14.51; P < .001) than did teeth without discoloration. Teeth with dark and yellow discoloration were significantly associated with pulp necrosis and pulp canal obliteration, respectively (P < .001). Conclusions This study demonstrates the importance of monitoring traumatized primary teeth with crown discoloration, particularly those appearing darker, owing to their higher risk of pulp necrosis and subsequent infection of the root canal system.

BackgroundDental fear affects children's oral health; there is, however, no evidence regarding the pathways linking possible predictors for its occurrence.AimThis study aimed at exploring the direct and indirect effects of sociodemographic, clinical, behavioural and psychosocial factors on the development of dental fear in schoolchildren over time.DesignThis is a prospective cohort study, with 10 years of follow‐up. The first assessment started in 2010 (T1) with a sample of 639 children aged 1–5 years from Southern Brazil. For this study, the follow‐up (T2) of these individuals was performed in 2020. Dental fear was assessed at T2 using the Brazilian version of the Children's Fear Survey Schedule–Dental Subscale (CFSS–DS). Socio‐economic, demographic, psychosocial and oral health measures were collected in both assessments. A structural equation model was performed to assess the direct and indirect pathways among variables at T1 and T2 to predict the CFSS–DS scores at T2.ResultsOf the 639 children, 429 were re‐evaluated at T2. Higher levels of untreated dental caries, younger individuals, dental visits for nonroutine reasons and low sense of coherence at T2 directly impacted dental fear at T2. Considering the indirect effects, the presence of dental caries and low household income at T1 indirectly impacted higher levels of dental fear over 10 years via dental caries at follow‐up.ConclusionOur findings suggest that socio‐economic, demographic, clinical, psychosocial and behavioural conditions can influence dental fear from childhood to adolescence.

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Objectives Evaluate the moderating effect of the SOC in the relationship between racial discrimination and OHRQoL in scholars. Materials and Methods This is a cross-sectional study nested in a cohort performed in southern Brazil. OHRQoL was assessed using the short version of the Child Perceptions Questionnaire 11–14. The perception of racial discrimination was measured using a question contained in the Bullying Questionnaire by Olweus, and SOC through the shortened version of the 13-item Sense of Coherence Scale. Sociodemographic and dental caries characteristics were also collected. A simple slop test and Poisson regression analysis were performed to test the interaction effects of the predictors on OHRQoL. The results are presented in Rate Ratio and 95% confidence interval. Results A total of 429 scholars were included in this study. About 6.7% reported had perceived racial discrimination. The simple slope test indicated that the negative effects of racial discrimination on OHRQoL were significant under different SOC levels. Among scholars who suffered racial discrimination, those who had higher SOC reported lower impact on OHRQoL (RR 0.70; 95%CI 0.55–0.89) when compared to those with low SOC. Conclusion SOC can be considered a moderating variable in the relationship between racial discrimination and OHRQoL. These findings highlight the potential importance of the SOC in reducing the harmful effects of racial discrimination on OHRQoL. Clinical Relevance: It is important that we present ways to minimize the impacts of racial discrimination in order to reduce the effects of these acts on different oral health outcomes throughout life.