Objective: Germline loss-of-function mutations in DEPDC5, and in its binding partners (NPRL2/3) of the mammalian target of rapamycin (mTOR) repressor GATOR1 complex, cause focal epilepsies and increase the risk of sudden unexpected death in epilepsy (SUDEP). Here, we asked whether DEPDC5 haploinsufficiency predisposes to primary cardiac defects that could contribute to SUDEP and therefore impact the clinical management of patients at high risk of SUDEP. Methods: Clinical cardiac investigations were performed in 16 patients with pathogenic variants in DEPDC5, NPRL2, or NPRL3. Two novel Depdc5 mouse strains, a human HA-tagged Depdc5 strain and a Depdc5 heterozygous knockout with a neuron-specific deletion of the second allele (Depdc5 c/À ), were generated to investigate the role of Depdc5 in SUDEP and cardiac activity during seizures. Results: Holter, echocardiographic, and electrocardiographic (ECG) examinations provided no evidence for altered clinical cardiac function in the patient cohort, of whom 3 DEPDC5 patients succumbed to SUDEP and 6 had a family history of SUDEP. There was no cardiac injury at autopsy in a postmortem DEPDC5 SUDEP case. The HA-tagged Depdc5 mouse revealed expression of Depdc5 in the brain, heart, and lungs. Simultaneous electroencephalographic-ECG records on Depdc5 c/À mice showed that spontaneous epileptic seizures resulting in a SUDEP-like event are not preceded by cardiac arrhythmia. Interpretation: Mouse and human data show neither structural nor functional cardiac damage that might underlie a primary contribution to SUDEP in the spectrum of DEPDC5-related epilepsies.

Abstract. Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis (MTB). The spread of MTB through the air is related to people's activities according to age, gender and occupation. The diagnosis of TB is currently enforced by the Molecular Rapid Test (TCM) method which replaces the microscopic examination of Acid-Fast Bacilli (AFB). The purpose of this study was to determine the relationship between the characteristics of pulmonary TB sufferers and the results of the TCM examination. This research is an analytic observational using secondary data. The subjects of this study were pulmonary TB patients at the Kertasemaya Health Center in the period January 2020 to December 2022. The research data included age, gender, occupation and TCM results. Analysis of the relationship between variables using the Chi-square test. A total of 100 subjects who met the inclusion criteria were patients diagnosed with pulmonary TB, the characteristics and results of TCM were recorded in medical records, and the age of the patient was over 15 years. The main characteristics of pulmonary TB sufferers are productive age (15-50 years) totaling 70 people (70%), male gender numbering 64 people (64%) and the majority not working totaling 57 people (57%). There was no relationship between age and TCM results (p=0.571), gender and TCM results (p=0.546), and occupation and TCM results (p=1.000). The conclusion of this study is that there is no relationship between age, gender, and occupation with the TCM examination results in pulmonary TB patients. Keywords: Age, Gender, Occupation, Pulmonary TB, TCM Abstrak. Tuberkulosis (TB) merupakan penyakit yang disebabkan Mycobacterium tuberculosis (MTB). Penyebaran MTB melalui udara berkaitan dengan aktivitas orang sesuai dengan usia, jenis kelamin, dan pekerjaan. Diagnosis TB saat ini ditegakkan dengan metode Test Cepat Molekuler (TCM) yang menggantikan pemeriksaan mikroskopik Basil Tahan Asam (BTA). Tujuan penelitian ini yaitu untuk mengetahui hubungan karakteristik penderita TB Paru dengan hasil pemeriksaan TCM. Penelitian merupakan observasional analitik menggunakan data sekunder. Subjek penelitian ini adalah pasien TB Paru di Puskesmas Kertasemaya pada periode Januari 2020 sampai Desember 2022. Data penelitian meliputi usia, jenis kelamin, pekerjaan serta hasil TCM. Analisis hubungan antar variabel menggunakan uji Chi-square. Total subjek berjumlah 100 orang yang memiliki kriteria inklusi yaitu pasien terdiagnosis TB Paru, Karakteristik dan hasil TCM tercatat rekam medis, dan usia penderita di atas 15 tahun.  Karakteristik utama penderita TB Paru adalah berusia produktif (15 – 50 tahun) berjumlah 70 orang (70%), jenis kelamin laki – laki berjumlah 64 orang (64%) dan mayoritas tidak bekerja berjumlah 57 orang (57%). Tidak ada hubungan antara usia dengan hasil TCM (p=0,571), jenis kelamin dengan hasil TCM (p=0,546), dan pekerjaan dengan hasil TCM (p=1,000). Kesimpulan penelitian ini adalah tidak terdapat hubungan antara usia, jenis kelamin, dan pekerjaan dengan hasil pemeriksaan TCM pada penderita TB paru. Kata Kunci: Jenis Kelamin, Pekerjaan, TCM, TB Paru, Usia

Abstract. Chronic suppurative otitis media or CSOM is an chronic inflammatory process that occurs in the middle ear, which is caused by bacteria with perforation of the tympanic membrane and the presence of ottorhoea. The most common causative bacteria is Pseudomonas aeruginosa. Topical antibiotic using ciprofloxacin can be used to treat for CSOM. Several studies have shown topical antibiotic to be more effective than systemic antibiotics. The aim of this study was to analyze the effectiveness of ciprofloxacin in the treatment of chronic suppurative otitis media. The study was conducted using systematic review method and database used in this study were Google Scholar, Science Direct, Proquest, Springer Link, Cochrane Library, and EBSCO HOST with 599 articles of journals obtained. The screening result on articles in accordance with the inclusion citeria were 344 articles and the exclusion criteria were 340 articles. The results of critical appraisal as many 4 articles.The study was conducted during March–December 2020. The results of review dan analysis from 4 articles of each study, topical ciprofloxacin was more effective topical neomycin, framycetin gramicidin-dexamethasone (FGD), and boric acid in the treatment of chronic suppurative otitis media. The parameters for relieving of symptomatic symptoms, perforation of tympanic membrane, and hearing quality. The conclusion of this study is that ciprofloxacin topical is more effective in the treatment of chronic suppurative otitis media. Abstrak. Otitis media supuratif kronik atau OMSK merupakan proses inflamasi kronik yang terjadi pada telinga bagian tengah yang diakibatkan oleh bakteri, ditandai dengan perforasi membran timpani serta ottorhoea yang terjadi secara terus menerus. Bakteri penyebab paling umum adalah Pseudomonas aeruginosa. Pengobatan OMSK dapat menggunakan antibiotik topikal siprofloksasin. Beberapa penelitian menunjukkan antibiotik topikal lebih efektif dibanding dengan antibiotik sistemik. Tujuan peneltian ini adalah menganalisis efektivitas siprofloksasin topikal pada pengobatan otitis media supuratif kronik. Penelitian ini merupakan systematic review dengan database yang digunakan adalah Google Scholar, Science Direct, Proquest, Springer Link, Cochrane Library, dan EBSCO HOST dengan jumlah artikel yang didapat sebanyak 599 artikel. Hasil skrining pada artikel yang sesuai dengan kriteria inklusi sebanyak 344 artikel dan kriteria eksklusi sebanyak 340 artikel. Setelah dilakukan telaah kritis, artikel yang di-review sebanyak empat artikel. Penelitian dilakukan pada periode Maret–Desember 2020. Hasil telaah dan analisis dari empat artikel menunjukkan bahwa siproflokasin topikal lebih efektif dibanding dengan topikal neomisin, framisetin gramisidin-deksametason (FGD), dan asam borik pada pengobatan otitis media supuratif kronik. Parameter kesembuhan gejala ottorhoea, perforasi membran timpani, serta kualitas pendengaran. Kesimpulan penelitian ini adalah siprofloksasin topikal efektif pada pengobatan otitis media supuratif kronik.

Paparan kebisingan di tempat kerja adalah salah satu bahaya yang paling sering terjadi. Diperkirakan 22,4 juta pekerja di seluruh dunia terpapar pada kebisingan yang berpotensi menyebabkan gangguan pendengaran akibat bising. Gangguan pendengaran akibat bising dapat dicegah dengan memakai ear plug. Penelitian ini bertujuan mengetahui hubungan kepatuhan penggunaan ear plug dengan keluhan gangguan pendengaran pada karyawan PT Anugrah Bungo Lestari. Penelitian ini bersifat deskriptif analisis dengan metode cross sectional. Variabel kepatuhan dan keluhan gangguan pendengaran diukur dengan menggunakan kuesioner yang diisi oleh responden. Sampel yang diteliti adalah pekerja bagian mesin dengan sampel sebanyak 100 responden dipilih secara total sampling dan dilakukan uji chi-square sebagai uji hipotesis. Penelitian dilakukan pada bulan September—Desember 2020 di PT Anugrah Bungo Lestari, Kabupaten Bungo, Provinsi Jambi. Hasil penelitian ini menunjukkan sebanyak 80% pekerja PT Anugrah Bungo Lestari sudah patuh dalam menggunakan ear plug. Terdapat pekerja yang mengeluhkan gangguan pendengaran, 45% pekerja mengeluhkan gangguan pendengaran tidak mengganggu aktivitas, 24% pekerja mengalami keluhan gangguan pendengaran yang mengganggu aktivitas, sedangkan sisanya tidak mengeluhkan gangguan pendengaran. Hasil uji diperoleh pada pekerja yang tidak patuh menggunakan ear plug terdapat keluhan gangguan pendengaran (p<0,001). Simpulannya adalah terdapat hubungan penggunaan ear plug dengan keluhan gangguan pendengaran. The Compliance Relationship of Using Ear Plugs to Complaints of Hearing Loss among Employees PT Anugrah Bungo LestariNoise exposure in the workplace is one of the most frequent hazards. It is estimated that 22.4 million workers worldwide are exposed to noise levels that have the potential to have noise induced hearing loss. Noise induced hearing loss can be prevented by wearing ear plugs. This study aims to determine the relationship of compliance with the use of ear plugs to complaints of hearing loss among employees of PT Anugrah Bungo Lestari. This research is descriptive analytic with cross sectional design. The compliance and hearing loss complaints variables were measured using a questionnaire filled out by the respondents. The sample studied were workers in the machine part, with a sample of 100 respondents chosen by total sampling and performed chi-square test as a hypothesis test. The research was conducted in September—December 2020 at PT Anugrah Bungo Lestari, Bungo Regency, Jambi Province. Resulting of this study indicate as many as 80% employees PT Anugrah Bungo Lestari was already obedient in using ear plugs. There are workers who complain about hearing loss, 45% of workers had complaints of hearing loss but did not interfere with activities, 24% of workers have complaints of hearing loss and interfere with activities, while the rest do not complain about hearing problems. The test results were obtained in workers who did not comply with using ear plugs who had complaints of hearing loss (p <0.001). The conclusion is that there is a relationship between the use of ear plugs and complaints of hearing loss.

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