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Four silages were prepared at 1-week intervals from a primary growth of timothy-meadow fescue sward. They were offered to 32 Finnish Ayrshire cows and supplemented with 7 or 10 kg of concentrate containing 0 or 1·15 kg rapeseed meal (RSM). The basal concentrate consisted of barley, oats and molassed sugar-beet pulp (2: 2: 1) and minerals. Diets were arranged 4 × 2 × 2 factorially in a cyclic change-over experiment with four periods of 21 days each.Silages contained 739, 730, 707 and 639 g digestible organic matter (D value) per kg dry matter (DM) for harvests I, II, III and IV, respectively. All dietary factors had marked effects on the performance of the cows but generally interactions between treatments could not be detected. A decrease of 10 g/kg in silage D value decreased energy-corrected milk (ECM) production of the cows by 0·50 kg/day and silage DM intake by 0·162 kg/day. An increment of 1 kg concentrate DM decreased silage DM intake by 0·61 kg and yielded 0·55 kg more ECM per day. Inclusion of RSM into the concentrate increased daily ECM production by 1·7 kg and silage DM intake by 0·60 kg/kg RSM when substituting the basal concentrate. Milk yield and yields of milk constituents decreased curvilinearly with delayed date of harvest the difference being greatest between the last two cuts and thus closely reflected the changes in silage D value. Milk protein concentration increased as apparent digestibility (P < 0.001) and concentrate level in the diet increased (P < 0.001). Milk fat concentration was not affected by the dietary treatments.Marginal ECM production responses to estimated metabolizable energy (ME) intake were higher when intake was manipulated with the date of silage harvest (0·138 kg ECM per MJ additional ME) compared with increased concentrate feeding (0·103); highest response was achieved by RSM feeding (0·244). Additional intake of calculated amino acids absorbed from the small intestine (AAT) manipulated by silage harvest time resulted in the highest marginal response (0·59 g milk protein per g additional AAT), while increased concentrate feeding and inclusion of RSM were equal (0·50 v. 0·49, respectively). Improving silage quality by earlier harvest resulted in higher milk yield, and in greater efficiency in the use of increments of estimated ME and AAT than was seen with increased concentrate feeding. Protein supplementation improved milk production irrespective of silage harvest date.

Copyright ? 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.Diets based on red clover silage (RCS) typically increase the concentration of polyunsaturated fatty acids (PUFA) in ruminant milk and meat compared with grass silages (GS), an effect that has been attributed to higher activity of polyphenol oxidase in red clover, promoting ruminal escape of dietary lipid. Four multiparous Finnish Ayrshire cows in mid lactation fitted with rumen cannulas were used in a 4?4 Latin Square design with 21-d experimental periods to evaluate the effects of incremental replacement of GS with RCS on ruminal lipid metabolism, using the omasal sampling technique in combination with Cr-EDTA, Yb acetate, and indigestible neutral detergent fiber as markers. Treatments comprised total mixed rations offered ad libitum containing 600 g of forage/kg of diet dry matter, with RCS replacing GS in a ratio of 0:100, 33:67, 67:33, and 100:0 on a dry matter basis. Silages contained a high proportion of lipid as nonesterified fatty acids (NEFA), with no difference between forage species (75 and 73% for GS and RCS, respectively). Substitution of GS with RCS had no influence on the intakes of NEFA, polar lipid, triacylglycerol, diacylglycerol, monoacylglycerol, or total fatty acids (FA), but altered the ingestion of specific FA. Replacing GS with RCS decreased linearly 18:3n-3 and increased linearly 18:2n-6 intakes. Changes in the proportion of RCS in the diet had no effect on the amounts or on the relative proportions of different lipid fractions at the omasum. On average, NEFA, polar lipid, triacylglycerol, diacylglycerol, and monoacylglycerol accounted for 80, 12, 4.4, 2.4, and 0.8% of total FA in omasal digesta, respectively. Replacement of GS with RCS increased linearly the amount of esterified and nonesterified 18:3n-3 at the omasum. Flows of cis-9 18:1 and 18:2n-6 were also increased linearly in response to RCS in the diet, whereas 3,7,11,15-tetramethyl-16:0 at the omasum was decreased. Replacing GS with RCS in the diet decreased linearly the lipolysis of dietary esterified lipids in the rumen from 85 to 70%. Effects on lipolysis due to forage species were also associated with linear decreases in apparent ruminal 18:3n-3 biohydrogenation from 93 to 85% and a trend toward lowered biohydrogenation of cis-9 18:1 and 18:2n-6 in the rumen. However, forage species had no effect on the flow of bound phenols formed as a consequence of polyphenol oxidase activity at the omasum. In conclusion, despite minimal differences in the extent of lipolysis in silo, lipid and constituent FA in RCS were less susceptible to ruminal lipolysis and biohydrogenation compared with GS.Peer reviewe

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Diets based on red clover silage (RCS) typically increase the concentration of polyunsaturated fatty acids (PUFA) in ruminant meat and milk and lower the efficiency of N utilization compared with grass silages (GS). Four multiparous Finnish Ayrshire cows (108 d postpartum) fitted with rumen cannulas were used in a 4 × 4 Latin square design with 21-d periods to evaluate the effect of incremental replacement of GS with RCS on milk production, nutrient digestion, whole-body N metabolism, and milk fatty acid composition. Treatments comprised total mixed rations offered ad libitum, containing 600 g of forage/kg of diet dry matter (DM), with RCS replacing GS in ratios of 0:100, 33:67, 67:33, and 100:0 on a DM basis. Intake of DM and milk yield tended to be higher when RCS and GS were offered as a mixture than when fed alone. Forage species had no influence on the concentration or secretion of total milk fat, whereas replacing GS with RCS tended to decrease milk protein concentration and yield. Substitution of GS with RCS decreased linearly whole-tract apparent organic matter, fiber, and N digestion. Forage species had no effect on total nonammonia N at the omasum, whereas the flow of most AA at the omasum was higher for diets based on a mixture of forages. Replacing GS with RCS progressively lowered protein degradation in the rumen, increased linearly ruminal escape of dietary protein, and decreased linearly microbial protein synthesis. Incremental inclusion of RCS in the diet tended to lower whole-body N balance, increased linearly the proportion of dietary N excreted in feces and urine, and decreased linearly the utilization of dietary N for milk protein synthesis. Furthermore, replacing GS with RCS decreased linearly milk fat 4:0 to 8:0, 14:0, and 16:0 concentrations and increased linearly 18:2n-6 and 18:3n-3 concentrations, in the absence of changes in cis-9 18:1, cis-9, trans-11 18:2, or total trans fatty acid concentration. Inclusion of RCS in the diet progressively increased the apparent transfer of 18-carbon PUFA from the diet into milk, but had no effect on the amount of 18:2n-6 or 18:3n-3 at the omasum recovered in milk. In conclusion, forage species modified ruminal N metabolism, the flow of AA at the omasum, and whole-body N partitioning. A lower efficiency of N utilization for milk protein synthesis with RCS relative to GS was associated with decreased availability of AA for absorption, with some evidence of an imbalance in the supply of AA relative to requirements. Higher enrichment of PUFA in milk for diets based on RCS was related to an increased supply for absorption, with no indication that forage species substantially altered PUFA bioavailability.

Mown herbage of timothy-meadow fescue (dry matter 218 (LDM) or 539 (HDM) g kg À1 ) was ensiled in laboratory silos to evaluate silage additives. For LDM silage, additives including formic acid (a blend of formic acid, sodium formate, propionic acid, benzoic acid, glycerol and another blend of formic acid and ammonium formate, both applied at 5 L t À1 ) were able to restrict fermentation and thereby improve intake potential of the silage. Aerobic stability (AS) of total mixed ration (TMR) was also improved. LDM grass treated with homofermentative lactic acid bacteria ( ho LAB) resulted in silage containing lactic acid at 132 g kg À1 DM, ammonium-N <40 g kg À1 total N, and pH < 3Á8, and the AS was poor (<36 h). The treatment including heterofermentative strain (Lactobacillus brevis) produced more acetic acid and better AS than ho LAB. Salt treatment (sodium benzoate, potassium sorbate, sodium nitrite) reduced pH compared to the Control treatment (3Á89 vs. 4Á24) and improved AS of TMR. The LDM Control silage had good AS, but the TMR based on it had poor AS. All additives were able to lower pH on HDM silages also, but other benefits of using additives were minimal. The treatment including L. brevis on HDM was able to improve AS of TMR.

The study was conducted to evaluate effects of silage type (grass-red clover vs. pure grass) and grain supplement (oats vs. barley) on rumen fermentation, post-ruminal nutrient flows, diet digestion and milk production. Four primiparous Finnish Ayrshire cows fitted with cannulae in the rumen and duodenum were used in a 4 × 4 Latin square experiment with four 28-d experimental periods and 2 × 2 factorial arrangements of treatments. Using red clover-containing (40%) silage rather than pure grass silage had minor effects on rumen fermentation or diet digestion but increased non-ammonia nitrogen (N) flow in terms of increased flows of microbial and dietary N entering to the small intestine. This was reflected as a reduced ruminal N degradability on grass-red clover diets. Furthermore, grass-red clover diets in comparison to grass silage diets increased milk lactose concentration and yields of milk, protein and lactose. Feeding oats in replacement for barley had minor effects on rumen fermentation or post-ruminal non-ammonia N flows but reduced digestibility of organic matter and neutral detergent fibre in the diet. Using oats rather than barley increased yields of milk and lactose but reduced milk protein concentration. Oats also increased proportions of C 18:0 and C 18:1 in milk fat and reduced those of C 10:0 to C 16:0 . It is concluded that inclusion of red clover and replacement of barley with oats in grass silage based diets have beneficial effects in dairy cow production.

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