Recebido em 30/1/04; aceito em 30/6/04; publicado na web em 5/11/04 RARE EARTHS: INDUSTRIAL AND BIOLOGICAL APPLICATIONS. The history of the rare earths is rich in innovation and these elements have been the object of study of a number of scientists. Rare earths are used practically in almost all aspects of life and these applications are due to their outstanding properties, mainly spectroscopic and magnetic. In industry, the applications of rare earths are many, such as in catalysis, phosphors, magnetism, glass and lasers. In biological systems, rare earths are used, for example, as luminescent probes in the investigation of binding sites in proteins, labels in immunoassays and in noninvasive tests.Keywords: rare earths; spectroscopy; medical applications. INTRODUÇÃOAs terras raras, para as quais se utiliza o símbolo Ln, correspondem aos elementos do lantânio (La, Z = 57) ao lutécio (Lu, Z = 71), entre os quais se incluem o ítrio (Y, Z = 39) e o escândio (Sc, Z = 21). Mas, segundo recomendações da IUPAC 1 , usam-se os termos lantanídeos para designar os elementos do La ao Lu e terras raras quando aos lantanídeos são incluídos o Sc e o Y.A expressão terras raras é imprópria para designar estes elementos, que receberam esta denominação porque foram inicialmente conhecidos em forma de seus óxidos, que se assemelham aos materiais conhecidos como terras. Além da expressão "terras" não ser apropriada à denominação de tais elementos, a expressão "raras" também não está de acordo, pois os lantanídeos são mais abundantes (com exceção do promécio que não ocorre na natureza) do que muitos outros elementos. Por exemplo, os elementos túlio (0,5 ppm) e lutécio (0,8 ppm) que são as terras raras menos abundantes na crosta terrestre, são mais abundantes que a prata (0,07 ppm) e o bismuto (0,008 ppm) 2,3 . O primeiro elemento das terras raras descoberto foi o cério, em 1751, pelo mineralogista suíço A. F. Cronstedt, quando obteve um mineral pesado, a cerita. Porém, existem controvérsias quanto a este fato e atribui-se o ano de 1787 como o início da história das terras raras, quando Carl Axel Arrhenius encontrou um mineral escuro, a iterbita (também conhecido como gadolinita), em uma pequena vila, Ytterby, próxima a Estocolmo 2 . Por constituírem uma família que apresenta propriedades físicas e químicas semelhantes, exigindo um trabalho imenso para separá-los com a obtenção de espécies relativamente puras, este grupo de elementos foi pouco explorado durante anos e somente em 1907 é que praticamente todas as terras raras naturais foram conhecidas 4-9 . A industrialização das terras raras teve início com a fabricação de camisas de lampiões. Com o passar do tempo suas propriedades foram tornando-se mais conhecidas e seus compostos passaram a ser mais utilizados, tais como na produção de "mischmetal" para pedras de isqueiro, baterias recarregáveis e aplicações metalúrgicas 5,10 . Com o desenvolvimento tecnológico as terras raras passaram a ganhar novos usos e, hoje em dia, o universo de suas aplicações é muito abrangente, sendo utiliz...
Proteins are the major constituents of muscle and are key molecules regulating the metabolic changes during conversion of muscle to meat. Brazil is one of the largest exporters of beef and most Brazilian cattle are composed by zebu (Nellore) genotype. Bos indicus beef is generally leaner and tougher than Bos taurus such as Angus. The aim of this study was to compare the muscle proteomic and phosphoproteomic profile of Angus and Nellore. Seven animals of each breed previously subjected the same growth management were confined for 84 days. Proteins were extracted from Longissimus lumborum samples collected immediately after slaughter and separated by two-dimensional electrophoresis. Pro-Q Diamond stain was used in phosphoproteomics. Proteins identification was performed using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Tropomyosin alpha-1 chain, troponin-T, myosin light chain-1 fragment, cytoplasmic malate dehydrogenase, alpha-enolase and 78 kDa glucose-regulated protein were more abundant in Nellore, while myosin light chain 3, prohibitin, mitochondrial stress-70 protein and heat shock 70 kDa protein 6 were more abundant in Angus (P<0.05). Nellore had higher phosphorylation of myosin regulatory light chain-2, alpha actin-1, triosephosphate isomerase and 14-3-3 protein epsilon. However, Angus had greater phosphorylation of phosphoglucomutase-1 and troponin-T (P<0.05). Therefore, proteins involved in contraction and muscle organization, myofilaments expressed in fast or slow-twitch fibers and heat shock proteins localized in mitochondria or sarcoplasmic reticulum and involved in cell flux of calcium and apoptosis might be associated with differences in beef quality between Angus and Nellore. Furthermore, prohibitin appears to be a potential biomarker of intramuscular fat in cattle. Additionally, differences in phosphorylation of myofilaments and glycolytic enzymes could be involved with differences in muscle contraction force, susceptibility to calpain, apoptosis and postmortem glycolysis, which might also be related to differences in beef quality among Angus and Nellore.
Twenty-four pregnant Nellore cows were randomly assigned into 2 feeding level groups (control [CTL]; fed 1.0 times the maintenance requirement; n = 12; and overnourished [ON]; fed at 1.5 times the maintenance requirement; n = 12) to evaluate effects of maternal overnutrition on fetal skeletal muscle development. Cows were slaughtered at 135, 190, and 240 d of gestation and samples of fetal LM were collected for analysis of mRNA expression analysis and for histological evaluation of collagen content and number of muscle cells. There was no interaction between gestational period and maternal nutrition for the variables evaluated (P > 0.05). The mRNA expression of Cadherin-associated protein, β 1 (β-catenin) tended to be greater in fetuses from ON cows (P = 0.08), while myogenic differentiation 1 (MyoD; P = 0.56), myogenin (MyoG; P = 0.70), and the number of muscle cells (P = 0.90) were not affected by maternal overnutrition. Gestational period did not affect the mRNA expression of β-catenin (P = 0.60) and MyoG (P = 0.21). The mRNA expression of MyoD tended to increase with days of gestation (P = 0.06). The mRNA expression of zinc finger protein 423 (Zfp423; P < 0.0001), C/EBPα (P = 0.01), and PPARγ (P < 0.0001) were enhanced in ON fetuses. No effects of days of gestation were observed for mRNA expression of Zfp423 (P = 0.75) and C/EBPα (P = 0.48). The mRNA expression of PPARγ in fetuses at 190 d of gestation tended to be greater than those at 135 and 240 d of gestation (P = 0.06). The mRNA expression of transforming growth factor β (TGF-β; P < 0.0001), collagen type III, α I (COL3A1; P < 0.0001), and collagen content (P = 0.01) were increased in ON fetuses. Gestational period did not affect the mRNA expression of collagen type I, α I (COL1A1; P = 0.65). The mRNA expression of COL3A1 (P = 0.09) in fetuses at 190 d of gestation tended to be greater than fetuses at 135 and 240 d of gestation. The mRNA expression of TGF-β in fetuses at 190 d of gestation was greater than in fetuses at 135 d of gestation (P = 0.03), and the values observed in fetuses at 240 d of gestation did not differ from the other gestational time points. The least value of collagen content (P = 0.01) was observed in fetuses at 135 d of gestation, and no differences were observed among the other gestational time points. These data shows that maternal overnutrition enhances fibrogenesis and likely adipogenesis without compromising myogenesis in fetal skeletal muscle of cattle.
This study was developed aiming to evaluate the effects of maternal feed-restriction on development of gastrointestinal tract (GIT) of bovine fetus at different gestational stages. Feed-restricted cows were fed 1.2 times the maintenance level while the control group was fed ad libitum. Pregnant cows were slaughtered at 136, 189, 239, and 269 days of gestation and gastrointestinal tracts of the fetuses were evaluated. No effects of maternal nutrition on body weight (P ¼ 0.17) and body length (P ¼0.13) of the fetuses were observed. No major effects of feed restriction on GIT mass of the fetuses were observed (P ¼ 0.51). However, the weight of small intestine per unit of body weight was 11.24% greater (P ¼0.04) in fetuses from restricted dams. Additionally, the length of small intestine and its villi were 12.93% and 16.44% respectively greater (P o .001) in fetuses from restricted dams compared to those from non-restricted dams. These data indicates that maternal feed-restriction does not affect the development of most of fetal gastrointestinal parts besides small intestine which in turn increases its surface area as a response of maternal feed restriction.
Studies have shown that intramuscular adipogenesis and fibrogenesis may concomitantly occur in skeletal muscle of beef cattle. Thus, we hypothesized that the discrepancy of intramuscular fat content in beef from Nellore and Angus was associated with differences in intramuscular adipogenesis and fibrogenesis during the finishing phase. To test our hypothesis, longissimus muscle samples of Nellore (n = 6; BW = 372.5 ± 37.3 kg) and Angus (n = 6; BW = 382.8 ± 23.9 kg) cattle were collected for analysis of gene and protein expression, and quantification of intramuscular fat and collagen. Least-squares means were estimated for the effect of Breed and differences were considered at P ≤ 0.05. A greater intramuscular fat content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). No differences were observed for mRNA expression of lipogenic and lipolytic markers ACC, FAS, FABP4, SERBP–1, CPT–2, LPL, and ACOX (P > 0.05) in skeletal muscle of Nellore and Angus cattle. Similarly, no differences were observed in mRNA expression of adipogenic markers Zfp423, PPARγ, and C/EBPα (P>0.05) However, a greater PPARγ protein content was observed in skeletal muscle of Angus compared to Nellore cattle (P≤0.05). A greater abundance of adipo/fibrogenic cells, evaluated by the PDGFRα content, was observed in skeletal muscle of Angus than Nellore cattle (P≤0.05). No differences in fibrogenesis were observed in skeletal muscle of Angus and Nellore cattle, which is in accordance with the lack of differences in intramuscular collagen content in beef from both breeds (P>0.05). These findings demonstrate that difference in intramuscular fat content is associated with a slightly enhanced adipogenesis in skeletal muscle of Angus compared to Nellore cattle, while no difference in fibrogenesis.
| INTRODUC TI ONThe demand for animal products is increasing as the world population grows. Brazil is an important beef producer and global exporter so it is important to invest in breeding programs aiming at traits that can meet the increasing demand for beef and the requirements of a demanding consumer (Carvalho et al., 2016). Also, beef cattle producers need to invest in technologies to increase profitability and improve the quality of the production. Therefore, the beef industry needs to reward the producer by implementing certification programs to stimulate the producers to adopt practices that improve meat quality, such as castrating males, crossbreeding, feedlot finishing, and slaughtering of heifers with adequate subcutaneous and intramuscular fat deposition. In the last decades, Brazilian beef producers have been using increasingly precocious cattle, with greater carcass development to produce beef with desirable traits. In short, Brazilian producers utilize crossbreeding between B. indicus and Taurus breeds to improve lean yield and beef quality (Carvalho et al., 2016;Gama et al., 2013;Pereira et al., 2015).Carcass and beef quality are significantly affected by gender (Gagaoua et al., 2015;Weglarz, 2010). Steer and heifer have lower performance, higher subcutaneous and intramuscular fat content, and better quality beef compared to bull carcasses (Seideman, AbstractThe study evaluated the effect of gender status on carcass and meat quality of feedlot Angus × Nellore cattle. A total of 176 cattle, 20 months old, were confined for 190-days and assigned to four treatments: bulls, immunocastrated, steers, and heifers. Bulls had greater rib eye area and HCW (p = 0.0001). Heifers had increased fat thickness (p = 0.0001). Steers and heifers had higher marbling scores (p = 0.0001).There was interaction between gender and aging time for Warner-Bratzler Shear Force (p = 0.0002), L* (p = 0.0118), and b* (p = 0.0113) values of beef. The sensory panel results showed that beef from bulls had the lowest consumer overall acceptance (p = 0.0278). Especially, regardless tenderness, steers and immunocastrated beef were considered tender, independent of aging time. Beef produced by heifers, steers, and immunocastrated is considered to be of higher quality than bulls. Thus, it is may be an interesting alternative to produce high-quality beef than bulls, to attend the consumer demand for high-quality products. Additionally, the low fatty acids n6 levels and low n6:n3 ratio, high levels of CLA, MUFAs, and oleic acid suggests that the heifer meat is favorable for human health.
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