In the present study, antibacterial and antioxidant [1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity] activities of a weed, slender amaranth (Amaranthus viridis L.) were investigated. Extracts of different plant parts were prepared in n-hexane, chloroform and ethyl acetate. Antibacterial activity was measured by using 100 mg mL-1 concentration extracts against 4 deadly phytopathogenic bacterial species viz. Pseudomonas syringae Van Hall, Ralstonia solanacearum Smith, Erwinia carotovora (Jones), Holland and Xanthomonas axonopodis Hasse. In antioxidants assays, 10, 20 and 30 mg mL-1 extracts were used keeping DPPH as control. In these bioassays, ethyl acetate fraction of A. viridis leaf exhibited the best antibacterial and antioxidants activity. Ethyl acetate leaf fraction showed the highest inhibition zone diameter (IZD) where it caused 21 mm IZD against P. syringae and 19 mm IZD against E. carotovora. This extract also showed 22, 52 and 84% antioxidant activity at 10, 20 and 30 mg mL-1 concentrations, respectively. Previously there is no report available that describes antibacterial activity of root extract of A. viridis against P. syringae. Moreover, antioxidant activity of stem and root extracts in n-hexane, chloroform and ethyl acetate was investigated first time in the world. It was concluded that the biological activities observed during the present investigation may be due to the presence of bioactive constituents that can be harnessed as natural antibacterials and antioxidants.
HIGHLIGHTS Antifungal activity of Amaranthus viridis leaves was evaluated against five phyto-pathogenic fungi. Ethyl acetate leaf fraction reduced the fungal growth up to 48%. GCMS analysis revealed 1,2-Benzenedicarboxylic acid, mono (2-ethylhexyl) ester) showing 58.5% peak area.
Drought is a harmful and slow natural phenomenon that has significant effects on the economy, social life,agriculture and environment of the country. Due to its slow process it is difficult to study this phenomenon. RemoteSensing and GIS tools play a key role in studying different hazards like droughts. The main objective of the study wasto investigate drought risk by using GIS and Remote Sensing techniques in district Khushab, Pakistan. Landsat ETMimages for the year 2003, 2009 and 2015 were utilized for spatial and temporal analysis of agricultural andmeteorological drought. Normalized difference vegetation index (NDVI) Standardized Precipitation Index (SPI) andrainfall anomaly indices were calculated to identify the drought prone areas in the study area. To monitormeteorological drought SPI values were used and NDVI was calculated for agricultural drought. These indices wereintegrated to compute the spatial and temporal drought maps. Three zones; no drought, slight drought and moderatedrought were identified. Final drought map shows that 30.21% of the area faces moderate drought, 28.36% faces slightdrought while nearly 41.3% faces no drought situation. Drought prevalence and severity is present more in the southernpart of Khushab district than the northern part. Most of the northern part is not under any type of drought. Thus, anoverall outcome of this study shows that risk areas can be assessed appropriately by integration of various data sourcesand thereby management plans can be prepared to deal with the hazard.
Agriculture is greatly impacted by climate change, which can lead to situations of food security or insecurity at both regional and global levels. Pakistan is predicted to experience an area reduction and geographical shifting of major crops in the near future. In the present study, we assessed the potential future distribution of wheat and maize in Pakistan. Based on current locations of these crops, we ran a Maxent species distribution model to predict future changes in crop distributions. We used 58 presence records for wheat and 48 presence records for maize. The model simulated current and future climate change scenarios (RCP 4.5 and RCP 8.5) based on the CMIP5 model, MPI-ESM-LR. Results from our model showed a decline in production area, with a 30-35% reduction in wheat and a 23-36% reduction in maize in the year 2070, depending upon which climate change scenario was modelled (i.e. RCP 4.5 or RCP 8.5). The model predictions were highly accurate, with test AUC values of 0.88 for wheat and 0.89 for maize. A jackknife test for variable importance indicated that irrigation, precipitation seasonality and precipitation of the warmest quarter are the most important environmental variables determining the potential geographic distribution of the crops. Due to the varying severity and nature of climate impacts, adaptation strategies are needed. This study can aid policy makers in devising policies that can help reduce the threat of future food insecurity in the region.
In the present study, the antibacterial activity of Cornus macrophylla was examined. Organic solvent extracts of leaves were prepared using methanol, n-hexane, chloroform, and ethyl acetate. Antibacterial activity was examined by using a 100 mg/mL extract concentration. Penicillin was kept as a positive control while dimethyl sulfoxide was taken as a negative control. Methanolic extract exhibited a 21.5, 36.3, 25.3, and 23.7 mm inhibition zone diameter (IZD); n-hexane showed a 33, 40, 32.8, and 28.7 mm IZD; chloroform showed a 18.8, 29, 22.3, and 21.6 mm IZD; and ethyl acetate showed a 23.5, 30.2, 30, and 22.3 mm IZD against Erwinia carotovora, Pseudomonas syringae, Ralstonia solanacearum, and Xanthomonas axonopodis, respectively. The n-hexane extract revealed high antibacterial activity against all bacterial species as compared with methanolic, chloroform, and ethyl acetate extract. Gas Chromatography Mass Spectrometry (GC/MS) analysis of n-hexane extract depicted the presence of 55 compounds. Out of these compounds, one compound, identified as α-amyrin (Mol. wt = 426), exhibited the maximum peak area (32.64%), followed by A’-Neogammacer-22(29)-en-3-ol, acetate, (3.beta.,21.beta.)- (Mol. wt = 468) and β-amyrin (Mol. wt = 426) having peak areas of 25.97 and 6.77%, respectively. It was concluded that the antibacterial activity observed during the present investigation may be due to these compounds.
-In agriculture, weeds have inevitable importance because of reducing the crop growth and also affecting food quality. Although, synthetic herbicides are available to combat these weeds but during recent years there is a trend of using ecofriendly herbicides extracted from natural resources. Present investigation is a continuity of the research, which reported a natural herbicidal compound named as Ophiobolin A from a fungus, Drechslera rostrata. The fungus was incubated in a growth medium of known composition (minimal medium) up to 28 days and its metabolites were extracted with organic solvents. The compound showing bioactivity was purified with the help of Reversed Phase High Performance Liquid Chromatography (RPHPLC) and identified with the help of Spectroscopic techniques viz. Mass Spectroscopy (MS) (LRESIMS and HRESIMS) and Nuclear Magnetic Resonance Spectroscopy (NMR). The purified compound caused death of superficial leaf cells of Chenopodium album, a noxious weed of wheat. Present investigation concludes that the identified compound could be used as structural analogue alternative to synthetic herbicides to synthesize natural herbicides.Keywords: Chenopodium album, natural herbicide, metabolites. RESUMO -Na agricultura, as plantas daninhas têm uma importância fundamental, por reduzirem o crescimento das culturas e também alterarem a qualidade da comida. Apesar de os herbicidas sintéticos estarem à disposição para combatê-las, nos últimos anos tem-se observado a tendência de usar herbicidas sustentáveis
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.