EOSINOPHILE IN HUMAN SKIN HOMOGRAFTING
523close sequence of events and complete degeneration was attained about 10 days after inoculation. At the peak of the changes effected, the contrast between healthy and infected cultures was very striking. As is well known, eventually healthy cultures will also undergo degeneration and necrosis and the gross appearance of these changes may closely resemble the changes called cytopathogenic when referring to infected cultures. However, this normally happens several weeks after injury and destruction of cells in virus infected cultures takes place.Specificity of virus action was observed in several experiments, where specific antiserum from patients known to have a high antibody titer to vaccinia, was allowed to act on the virus and apparently had a decelerating and, in some cases, completely inhibitory action. This aspect of the study, and the question of survival and quantitation of the virus in supernatant fluids, is being studied at present.Conclusions. The cytopathogenic effect of vaccinia virus in roller tubes and Maximow double cover slip tissue cultures was established in its principal aspect of degenerative and pathological changes in cell morphology. Specificity of this effect was corroborated as far as possible at the present with coordinated serological tests and animal experiments. Further studies and analysis of visual changes by phase microscopy and photomicrography are planned. The revival of interest in skin homografting is evidenced by the increase in the number of papers which have appeared in the medical literature within the past several years. Many of these reports explain the sloughing of most skin homografts on the basis of an acquired *This investigation was .made possible by grants from the Atomic Energy ChmTnission and the Milbank Mearmrial Fund. Fresenitd at the Tissue Transplantation Conference, M e n House, HaJriman, N. Y., W. 7 d 8, 1952. t Mcilbank Fellow in Plastic Surgery (1951-1952).
(At present, Department of Surgery, Presbyterian Hospital and )College of Physicians and Surgeons,Cohmbia University, New York) . immunity reaction. In reviewing the literature( 1-4) it becomes apparent that skin homograf tings have been permanently successful only between identical twins ( 5 ) , or between members of the same family(6,7). That the compatibility of blood types between donor and recipient remains an important factor necessary for permanent survival of homografts is indicated by other recent reports (4,8,9). Extensive skin homograft research in animals has been described( 10-15). Brown and McDowell ( 16) found "many eosinophiles" in biopsies taken of the sloughing homograf t in split-thickness homograf ting. Split-thickness homografting in a child reat UNSW Library on July 23, 2015 ebm.sagepub.com Downloaded from
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