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To ascertain genetic diversity, population structure and linkage disequilibrium (LD) among a representative collection of Chinese winter wheat cultivars and lines, 90 winter wheat accessions were analyzed with 269 SSR markers distributed throughout the wheat genome. A total of 1,358 alleles were detected, with 2 to 10 alleles per locus and a mean genetic richness of 5.05. The average genetic diversity index was 0.60, with values ranging from 0.05 to 0.86. Of the three genomes of wheat, ANOVA revealed that the B genome had the highest genetic diversity (0.63) and the D genome the lowest (0.56); significant differences were observed between these two genomes (P<0.01). The 90 Chinese winter wheat accessions could be divided into three subgroups based on STRUCTURE, UPGMA cluster and principal coordinate analyses. The population structure derived from STRUCTURE clustering was positively correlated to some extent with geographic eco-type. LD analysis revealed that there was a shorter LD decay distance in Chinese winter wheat compared with other wheat germplasm collections. The maximum LD decay distance, estimated by curvilinear regression, was 17.4 cM (r2>0.1), with a whole genome LD decay distance of approximately 2.2 cM (r2>0.1, P<0.001). Evidence from genetic diversity analyses suggest that wheat germplasm from other countries should be introduced into Chinese winter wheat and distant hybridization should be adopted to create new wheat germplasm with increased genetic diversity. The results of this study should provide valuable information for future association mapping using this Chinese winter wheat collection.

Water scarceness is a major threat to wheat productivity under changing climate scenarios, especially in arid and semi-arid regions. However, growing drought-tolerant wheat genotypes could be a sustainable option to enhance wheat productivity under drought stress conditions. The aim of this study was to evaluate the effect of mild to severe drought stress on gas exchange parameters, relative water content, SPAD-chlorophyll value, and yield-related parameters of 14 wheat genotypes being cultivated in arid to semi-arid areas on large scale. The genotypes were grown in earthen pots under three drought levels, namely (1) control-well watered, (2) mild water stress, i.e., 60% water holding capacity, and (3) severe water stress, i.e., 40% water holding capacity. The drought was imposed from the jointing stage to physiological maturity. Drought significantly decreased net photosynthesis, stomatal conductance, relative water contents, 100-grain weight, and grain yield in all genotypes. However, the reduction percentage was different in different genotypes under drought stress compared with well-watered conditions. The highest relative water content (65.2%) was maintained by the genotype Galaxy-2013, followed by AAS-2011 (64.6%) and Johar-2016 (62.3%) under severe drought conditions. Likewise, Galaxy-2013 showed the highest net photosynthesis and stomatal conductance under severe drought conditions. The highest grain yield per plant (6.2 g) and 100-grain weight (3.3 g) was also recorded in Galaxy-2013 under severe drought conditions, while the highest grain yield under well-watered conditions was recorded in Johar-2016, followed by Galaxy-2013. These results suggest that wheat variety Galaxy-2013 could be cultivated extensively to obtain good wheat yield under limited water conditions.

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Carbon isotope discrimination (D) has been recognized as a valuable phenotyping tool in wheat breeding. However, technical expertise and analysis cost restrict its large-scale use. We examined the associations of ash content (AC), minerals content (Ca, K, Mg, Fe and Mn) and leaf chlorophyll content (Chl) with grain D and grain yield (GY) to assess their potential as substitute to grain D. We evaluated 49 wheat genotypes under two water deficit regimes (W 120 and W 200 ) in a rain-out shelter. Leaf chlorophyll content (Chl) was strongly correlated with grain D and GY under moderate water deficit regime (W 200 ). Significant and negative correlations (P \ 0.01) of AC and potassium concentration (K) with grain D, and between AC and GY was observed under both water regimes, while manganese concentration (Mn) was negatively correlated with grain D under W 120 regime only and magnesium concentration (Mg) correlated negatively under the W 200 regime only. Grain D was correlated (P \ 0.01) positively with photosynthesis rate (A), stomatal conductance (g s ) and GY, while correlated negatively (P \ 0.01) with intrinsic water use efficiency (iWUE) under both water regimes. Results confirm the role of grain D as an indirect selection criterion for drought tolerance under a wide range of drought conditions. Additionally, Chl is the most suitable trait to predict yield under moderate water deficit conditions. AC and K concentration in grain proved potentially useful and economical alternative criterion to grain D in the evaluation of differences in yield potential and drought tolerance in wheat under drought.

Soil salinity disrupts the physiological and biochemical processes of crop plants and ultimately leads to compromising future food security. Sodium nitroprusside (SNP), a contributor to nitric oxide (NO), holds the potential to alleviate abiotic stress effects and boost tolerance in plants, whereas less information is available on its role in salt-stressed lentils. We examined the effect of exogenously applied SNP on salt-stressed lentil plants by monitoring plant growth and yield-related attributes, biochemistry of enzymes (superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD)) amassing of leaf malondialdehyde (MDA) and hydrogen peroxide (H2O2). Salinity stress was induced by NaCl application at concentrations of 50 mM (moderate salinity) and 100 mM (severe salinity), while it was alleviated by SNP application at concentrations of 50 µM and 100 µM. Salinity stress severely inhibited the length of roots and shoots, the relative water content, and the chlorophyll content of the leaves, the number of branches, pods, seeds, seed yield, and biomass per plant. In addition, MDA, H2O2 as well as SOD, CAT, and POD activities were increased with increasing salinity levels. Plants supplemented with SNP (100 µM) showed a significant improvement in the growth- and yield-contributing parameters, especially in plants grown under moderate salinity (50 mM NaCl). Essentially, the application of 100 µM SNP remained effective to rescue lentil plants under moderate salinity by regulating plant growth and biochemical pathways. Thus, the exogenous application of SNP could be developed as a useful strategy for improving the performance of lentil plants in salinity-prone environments.

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Excessive tillage in conventional agriculture systems may cause plough pan, which alters soil physical properties, and thus adversely affects the crop growth and productivity. This study was conducted to monitor the effect of different tillage practices in wheat-based cropping systems on soil physical properties, allometry and grain yield of wheat. Wheat was planted in different cropping systems (viz. fallow-wheat, rice-wheat, cotton-wheat, mungbean-wheat and sorghum-wheat with zero tillage, conventional tillage, deep tillage and on two types of beds (60/30 cm with four rows) and (90/45 cm with six rows). Interaction between different tillage practices and cropping systems had significant effect on soil bulk density and total porosity, wheat allometry and grain yield. Minimum bulk density tied with higher total porosity was recorded in both types of bed sowing followed by deep tillage. This improvement in soil physical properties caused improvement in leaf area index and duration, specific leaf area, crop growth, and net assimilation rates. As a result, the productivity of bed sown wheat was better; however, grain yield of zero tilled wheat was low due to poor crop growth and net assimilation rate. Wheat productivity was substantially low when planted after sorghum; nonetheless, and was quite high when sown after mungbean. In crux, wheat planting on beds after mungbean is the best option considering the long-term environmental sustainability of wheat-based cropping systems.