The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is one of the most important fruit pests worldwide. Mediterranean fruit fly control in Spain has been based on organophosphate sprays, especially malathion, mixed with protein baits. However, this insecticide has recently been excluded from annex 1 of the Directive 91/414 CEE, which lists authorized active ingredients for pest control in the European Union. This article reports on the efficacy of four alternative baited insecticides on Mediterranean fruit fly and their side effects on three natural enemies [Cryptolaemus montrouzieri (Mulsant), Neoseiulus californicus (McGregor), and Aphidius colemani (Viereck)] relevant for pest control in citrus agroecosystems. A high Mediterranean fruit fly mortality was obtained for all baited insecticides (phosmet and spinosad) except lambdacyhalothrin, which caused the lowest mortality and showed a novel disabling effect on surviving Mediterranean fruit fly adults. Spinosad proved to be the most selective bait treatment for C. montrouzieri and N. californicus, whereas for A. colemani the most selective bait was phosmet and lambda-cyhalothrin. These findings would contribute to a sustainable chemical control of C. capitata populations under an integrated pest management system in Spanish citrus orchards.

Gut content analysis using molecular techniques can help elucidate predator-prey relationships in situations in which other methodologies are not feasible, such as in the case of trophic interactions between minute species such as mites. We designed species-specific primers for a mite community occurring in Spanish citrus orchards comprising two herbivores, the Tetranychidae Tetranychus urticae and Panonychus citri, and six predatory mites belonging to the Phytoseiidae family; these predatory mites are considered to be these herbivores' main biological control agents. These primers were successfully multiplexed in a single PCR to test the range of predators feeding on each of the two prey species. We estimated prey DNA detectability success over time (DS50), which depended on the predator-prey combination and ranged from 0.2 to 18 h. These values were further used to weight prey detection in field samples to disentangle the predatory role played by the most abundant predators (i.e. Euseius stipulatus and Phytoseiulus persimilis). The corrected predation value for E. stipulatus was significantly higher than for P. persimilis. However, because this 1.5-fold difference was less than that observed regarding their sevenfold difference in abundance, we conclude that P. persimilis is the most effective predator in the system; it preyed on tetranychids almost five times more frequently than E. stipulatus did. The present results demonstrate that molecular tools are appropriate to unravel predator-prey interactions in tiny species such as mites, which include important agricultural pests and their predators.

Further studies, like that presented here, are necessary to gain a better understanding of integrating biological and chemical controls. When considering both efficacy and side effects on beneficial arthropods, the best options would seem to be mineral oil, tebufenpyrad and clofentezine. However, it is urgent to complete testing of the side effects of the acaricides used in citrus. This question is crucial if the fact that two recently introduced Tetranychidae are being controlled in citrus by chemical means exclusively is considered.

Field surveys were conducted from 2004 to 2007 to determine the species composition and relative abundance of natural enemies associated with colonies of either the citrus red mite, Panonychus citri, or the two spotted spider mite, Tetranychus urticae, in Valencian citrus orchards (eastern Spain). Fourteen species were recorded, six phytoseiid mites and eight insect predators. Two of them are reported for the first time on citrus in Spain and two more are first reports as predators associated with T. urticae. The community of predators associated with T. urticae and P. citri was almost identical, and the Morisita-Horn index of similarity between both natural enemy complexes was close to one, suggesting that predators forage on both pest species. Quantifying the presence of many known spider mites predators in Valencian citrus orchards is an important first step towards spider mite control. A challenge for future studies will be to establish conservation and/or augmentation management strategies for these predators, especially to improve T. urticae biological control.

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Food waste (FW) has recently attracted the interest of different institutions and has been the focus of many studies due to its important environmental, social and economic impact. This paper aims to analyze whether a didactic intervention, consisting of informing teachers and pupils and involving pupils in reducing FW, could bring about changes in the level of knowledge and attitude towards FW and in the amount of FW generated during the mid-morning break and lunch at schools. This study was conducted at a public Primary School in Valencia (Spain). Subtle changes in the level of knowledge and attitude towards FW were detected in teachers and pupils after the intervention. Around 30% of FW reduction at lunch was observed in the intervention group but not in the other groups. A decrease of almost half of the average weight was observed during the mid-morning break in the rest of primary groups. The results apparently show that addressing the FW issue in classrooms can have a very positive effect on children’s attitudes. As it is such a cross-cutting issue; it raises awareness about a large group of Sustainable Development Goals, and encourages these young citizens to make conscious decisions and to act responsibly.

The management of Tetranychus urticae, a key pest of clementine trees, is mainly based on the use of acaricides. However, more environmentally safe measures, such as biological control, are being encouraged. Life-history traits of the three most abundant phytoseiid mites associated with T. urticae on this crop (Euseius stipulatus, Phytoseiulus persimilis and Neoseiulus californicus) were studied. The experiments were performed under laboratory conditions (25 degrees C, 80 +/- 5% RH and 16:8 h (L:D)) on clementine leaves and T. urticae as prey. Euseius stipulatus could not complete its life cycle, whereas P. persimilis and N. californicus completed it satisfactorily. The estimated intrinsic rate of increase (rm) was significantly higher for P. persimilis (0.344 day(-1)) than for N. californicus (0.244 day(-1)) and both were higher than the rm value of T. urticae on clementine leaves. Implications of these results for the biological control of T. urticae in this crop are discussed.

Petroleum-derived spray oils (PDSOs) offer an interesting alternative to acaricides to control the twospotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), a key pest of clementine mandarins, Citrus reticulata Blanco. However, there is a lack of knowledge on how these products should be used. In this study, we evaluated the efficacy of four PDSOs (Sunspray Ultrafine, Volck Miscible, Texaco D-C-Tron Plus, and Agroaceite) at five concentrations (0.5, 1.0, 1.5, 2.0, and 3.0%) against eggs, protonymphs, and adults of T. urticae. We also characterized the PDSOs deposition pattern to find out the possible relationship between this factor and efficacy. In general, for all PDSO assayed, the higher the concentration, the higher the coverage, the mean area of impacts and efficacy on T. urticae. The biggest mean area of the impacts corresponded to Texaco D-C-Tron Plus. This PDSO was the most effective one and its efficacy was independent of concentration for concentrations higher than 1.0%. The same applied for concentrations higher than 1.5-2.0% for Agroaceite, Volck Miscible, and Sunspray Ultrafine, with high efficacies against eggs, protonymphs, and adults. PDSOs are highly effective against T. urticae, the use of these products should be encouraged in integrated citrus pest management programs in Spain. The next step will be to ascertain the efficacy under real field conditions.